| Hyperlipidemia is a principal factor in Atherosclerosis (AS). Now cardiovascular disease caused by AS is one of diseases endangering human life. Hyperlipidemia is closely related to coronary heart disease (CHD), hypertension, brain vascular disease, diabetes, kidney disease and hypothyroidism.More than 40 kinds of antihyperlipidemic medicines have been developed in the world .But more studies were concentrated on two kinds, namely statins and fibrate, because their curative effects were established. Indications of statins is hypercholesterolemia, while that of fibrate is hypertriglyceridemia. Study showed, that antihyperlipidemic medicine can reduce mortality of heart disease and outbreak rate of nonlethal heart disease by 24% and 19% respectively. Antihyperlipidemic medicine not only lessen AS, but also can promote recovery.For now, hyperlipidemia has not been cured completely by any medicine. Some antihyperlipidemic medicines can cure hyperlipidemia partly with long term, which result in side effect . Clinically showed, that Piper Longum L., on which we study now, has significant antilipidic effect with no side effect.So, to separate the best antilipidic constituents from Piper Longum L., we have obtained a series of compounds, which have antilipidic effect.Piper Longum L. fruit was extracted with ethanol, and ethanol extract was concentrated, named PP1Rats and mice as experimental animals, verify high lipidic diet and antilipidic effect of PPL Result showed, that high lipidic diet can significantly increase serum TC level (normal vs. control, Rats, P=9.987E-08, Mice, P=2.829E-09), but has little effect on serum TG in rats and reduce the serum TG in mice, PP1 reduce serum TC (vs. control, rats, P=0.021, mice, P=0.053) and increase the serum TG in both rats and mice.PP1 was chromatographed over silica gel and eluted with a 1.5:1 mixture of hexane - ethylacetate, which afford 6 fractions, according to elution sequence, named F1, F2, F3, F4, F5 and F6, then eluted with ethanol and obtained F7.Rats as experimental animals, select the effective fraction. Result showed, that antilipidic effects of F3 and F4+5 (mixture of F4 and F5, because proportion of F4 is little and hardly separate pure F4) were better than others. Verifying pharmacological experiment showed, that F4+5 reduce serum TC (vs. control, P=0.002) and serum TG (vs. control, P=0.012), Simvastatin reduce serum TC (vs control, P=0.242) and increase serum TG.F4+5 was chromatographed over silica gel and eluted with a 1.5:1 mixture of hexane - ethyl acetate, which afford 3 fractions. 3 fractions were respectively chromatographed on the same condition, which obtained F4, F5(l) and F5(2)(main component of crude F6 by HPLC). F4 and F5(2)were recrystallized in methanol to yield white and yellow crystals. F4 and F5(2) were pure, and F5(l) was a mixture, which were confirmed by HPLC. Retention time of F5(2) is identical with that of Piperine, and F5(l) was composed of two big peaks and two small peaks, in which retention time of a big peak is identical with that of Piperine.Rats as experimental animals, study on antilipidic effect of F5(l). Result showed, that F5(l) and Simvastatin reduce serum TC (vs. control, F5(l), P=0.203, Simvastatin, P=0.226), reduce serum APOB (vs. control, F5(l), P=0.115, Simvastatin, P=0.355), increase HDL-C (vs. control, F5(l), P-0.149, Simvastatin, P=0.629), reduce serum LP(a) (vs. control, F5(l), P-0.056, Simvastatin, P=0.441), have little effect on serum TG, APOA and LDL-C.Rats as experimental animals, study on pharmacology of Piperine and Tween-80. Result showed, that Piperine reduce serum TC (vs. control, P=0.054), while Simvastatin has no effect on serum TC, Piperine reduce serum TG (vs. control, P=0.243) and Simvastatin significantly reduce serum TG (vs. control, P=0.039), Tween-80 slightly reduce serum TC (vs. control, P=0.543) and increase serum TG.F5(1) was separated by HPLC , and the biggest peak (retention time is 26.217min) was collected and Named F5(l1).Rats as experimental animals, study on pharmacolog...
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