Purification And Structure Investigation Of Polysaccharide From Spores Of Ganoderma Lucidum

Water-soluble crude polysaccharide has been extracted from spores of Ganoderma Lucidum. Analysis indicated that it contained glucose (Glc) and galacose (Gal), and its molar ratio is 16.53:1.00. The crude polysaccharide has been deproteinizationed by the combined methods of enzyme and Sevage, fractionated and purified by Sepharose CL-6B gel filtration. Three fractions: GLP₁,GLP₂,GLP₃ were got respectively. So GLP₁ was got .By gel filtration on Sepharose CL-6B, HPLC and specific optical rotation measurement test. GLP₁ was a single strait symmetry apex. The identification of purify implies that GLP1 was respectively uniform on molecular weight and polarity. GLP₁ was a gray white substance. G.C. analysis indicted that GLP₁ was only composed of Glc. Partial hydrolysis with acid and hydrolysis with fibrin enzyme, Periodate oxidation, Smith degradation, I.R., G.C., Methylation and 13C-NMR were used on the structure analysis of GLP₁. Methylation production were hydrolyzed, deoxidize, then analyzed by G.C.-M.S. The results revealed that GLP₁ was a kind of highly branched structure. The main chain was composed of (1→6) -linked-Glc, and (1→3)-linked-Glc residues were substituted at O-3, O-4. The results revealed the main chain of GLP₁ is made up of (1→6)-linked- Glc as well as substituted at 0-4 and 0-3. On average, there are 3 branches among 10 main chain residues. In vitro antitumor activities were investigated by MTT methods The results revealed that GLP₁ had no direct killing action for hela cells (P>0.05). The anti-tumor action of GLP₁ in mice was mediated by the corpus immune system.