| A water-soluble crude polysaccharide had been isolated from Carthamus tinctorius L.. After purified, a homogeneous polysaccharide CTP was obtained, then the investigations of composition, characterization, structure and activity had been done. Crude polysaccharides had been gotten by hot water extraction and ethanol precipitation. Content of polysaccharide was developed by the use of phenyl hydrate-sulfuric acid. Compositions were determined by GC analysis. After freezed-melted repeatedly, deprotinzed Sevag method, fractionated by ultration and gel chromatography, the water-soluble polysaccharide CTP was obtained. The homogeneity of CTP was proved by specific optical rotation measuremend and HPLC, etc. By means of HPLC, partial hydrolysis with acid, periodate oxidation, Smith degradation, GC, methylation and GC-MS analysis, 13CNMR spectroscopy, the structure of CTP was identified. The anti-tumor activity of CTP was detected in vitro by MTT. Analysis of PC and GC indicated that it contained Glc and Gal, and its molax ratio was 8.07:1. CTP was a homogeneous and neutral hetero-polysaccharide and its molecular weight was about 4000-5000, G.C. analysis indicated that it was composed of Glc and Gal in the molar ratio of 6.08:1. CTP was a kind of small molecular weight polysaccharides with many branches. The main chain of CTP was made up of (1→4)-linked-Glc with five side chains. They were (1→3,6),(1→6),(1→)-linked-Gal, (1→2)-linked-Glc, (1→6),(1→)-linked-Glc, and two (1→6)-linked-Glc respectively. The anti-tumor in vitro of CTP was unnotable. Carthamus tinctorius L. is a kind of Chinese traditional and herbal grugs. Polysaccharides as its effective ingredients have extensive pharmaceutic values, and it is worthy of advanced research.
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