Studies On The Technology Of Supercritical Carbondioxide Extraction Of Peucedanum Praeruptorum Dunn

8-methoxypsoralen ( 8-MOP ) and praeruptorin A ( Pd- I a ) in Peucedanum praeruptorum Dunn were identified by TLC with spot clear and reproducible. Quantitative assays were performed on the two active components of 8-MOP and Pd-I a by HPLC. Active components had good linearity with the range of 0.05 μ g～0.35 μ g(r=0.9996) and 0.2 μ g—1.8 μ g(r=0.9996) respectively. And the average recoveries of them were 98.0%(RSD=1.72%)and 98.3%(RSD=1.51%). The two methods can be used for the quality control.The effects of pressure, temperature, extraction time, the flow rate of CO₂, the CO₂ density, kinds of entrainer on the extractive results of Peucedanum praeruptorum Dunn were explored, at the same time , the curves of the influence factors were drawn. The optimum conditions for SFE- CO₂ was selected with the aid of orthogonal design by using the content of 8-MOP , Pd- I a and the yield of extractor as quality control criteria. The results were as followed: pressure 25MPa, temperature 60℃, extraction time 3h, 95%alcohol as entrainer (1 : 1 w/v) and the rate flow of CO₂ 3.2L/h. Compared with the technology of traditional solvent extraction, SFE-CO₂ of Peucedanum praeruptorum Dunn was proved to be efficient, simple and save the extraction time. Meanwhile the extractor of SFE-CO₂ has light color and...