Studies Of Bioactive Components Extracted Form Alga Using Supercritical Carbon Dioxide Extraction And Its Antioxidantive Activtity

Marine algae contains highly polysaccharides, phycobiliprotein and polyunsaturated fatty acid as well as pigments which are supposed to have the capacity of radicals scavenging, inhibition against lipid oxidation, tumor-preventing, blood circulation improving, antibiosis, anti-inflammatory, dermerto-protection and anti-aging as well. In our studies, Chlorella pyrenoidosa and Spirulina platensis with high antioxidant actives were screened out from chlorella, blue-green algae and brown algae. Optimization were made on the extraction techniques and extracting parameters of bioactive components from these two varieties of chlorella by SC-CO₂. In vitro antioxidant activities of SC-CO₂ extracts were investigated and the results were showed as followed:1. Soxhlet Extraction was performed to extract bioactive components from Chlorella pyrenoidosa, Spirulina and guljweed. The yield from Chlorella pyrenoidosa was 1.42±0.22% and radical scavenging capability was shown to be 97.8± 1.3%. However, yield from Spirulina was 1.18±0.23% and its radical quenching activity, 84.0± 0.3%. The yield from guljweed was 0.58±0.11% and its radical quenching activity, 41.7±0.5%. Based on the above results, Chlorella pyrenoidosa and Spirulina were therefore subject for investigation on yields and antioxidant acvitites of bioactive components.