Separation And Extraction Of Alkaloids From Chinese Traditional Medicine Using Emulsion Liquid Membrane

Alkaloids in Chinese Traditional Medicine have a variety ofmedicinal value because of their excellent biological activity. Commonseparation methods, liquid-liquid extraction, supercritical fluid extraction,ultrasonic wave extraction, microwave assisted extraction, acceleratedsolvent extraction, common ultra-high pressure extraction, macro-porousadsorption resin extraction and molecular imprinting extraction have beendeveloped to separate and extract bioactive components from ChineseTraditional Medicine. Emulsion liquid membrane, as a simple, quick,high-efficiency, high-selectivity and high-economy separation andextraction technique, has been widely used in the fields of environment,chemistry production, wet metallurgy and biological engineering.However, the reports about separation and extraction of active ingredientsfrom Chinese Traditional Medicine using emulsion liquid membrane arevery few due to the complex composition and multiinterferent exist. Thesystemic study has been carried out in the present paper onseparation andextraction of alkaloids from Chinese Traditional Medicine using emulsionliquid membrane. The details are as follows:1. A literature review is made on the regular separation and extractionmethods of alkaloids from Chinese Traditional Medicine which appearin recent years, Then, the emulsion liquid membrane is introduced indetail.2. The separation and extraction of aporphine alkaloids, N-nornuciferine,O-nornuciferine and nuciferine in the lotus leaf have been carried outusing emulsion liquid membrane of D2EHPA-Span 80- kerosene. Under the optimized experimental conditions, the extraction rates ofN-nornuciferine, O-nornuciferine and nuciferine in the lotus leaf reach95.6ï¼…, 100ï¼…, and 97.9ï¼…, respectively. The enrichment factors are8.73, 8.50, and 8.04, respectively. The results indicate that the presentemulsion liquid membrane system can extract and separate effectivelythese three kinds of alkaloids from lotus leaf.3. A systematic research on the extraction and enrichment of theprotopine, allocryptopine, sanguinarine, and chelerythrine in theMacleaya Cordata (Willd) R. Br. with the emulsion liquid membranesystem of D2EHPA- Span 80- kerosene. Under the optimizedexperimental conditions, the extraction rates of protopine,allocryptopine, sanguinarine and chelerythrine in the macleaya cordata(Willd) R. Br reach 80.4ï¼…, 77.9ï¼…, 88.3ï¼…, and 75.6ï¼…, respectively.The enrichment factors of these four alkaloids are 8.57, 8.31, 9.42, and8.06, respectively. The results indicate that the present emulsion liquidmembrane system can extract and separate these four kinds ofalkaloids from the Macleaya cordata (Willd) R. Br. very well.4. In view of green chemistry, the poisonless edible tea oil was selected asthe membrane solvet instead of kerosene. The research has beencarried out on the extraction and enrichment of the protopine,allocryptopine, sanguinarine, and chelerythrine in the Macleayacordata (Willd) R. Br. with the emulsion liquid membrane system ofD2EHPA-Span 80-n-decanol-edible tea oil. Under the optimizedexperimental conditions, the extraction rates of protopine,allocryptopine, sanguinarine and chelerythrine in the Macleayacordata(Willd) R. Br. reach 81.1ï¼…, 77.8ï¼…, 98.4ï¼…, and 78.7ï¼…, respectively. The enrichment factors of these four alkaloids are 10.9,10.5, 18.2, and 16.2, respectively. Comparing the system usingkerosene as the membrane solvent, the extraction rates and theenrichment factors of alkaloids are obvious higher. Moreover, the timeof breaking the milk reduces greatly in the present system. The resultsdemonstrate that the improved membrane system has better function.