Extraction, Separation And Purification Of Active Ingredients Group From Yupingfeng Compound

Yupingfeng compound composing of Astragali, Atractylodis macrocephala andSanposhnikoviae, is recorded in Dan Xi Xin Fa written by Zhu Dan-xi in dynasty Yuan, whichis a classic famous prescription medicine extensively used in clinical. Separation andpurification of the active ingredients group from Yupingfeng were studied, and the results areas follows:(1) Analysis methods for the determination of astragalosideⅣwith SPE-HPLC anddetermination of astragaloside with ultraviolet-visible absorption spectroscopy wereestablished.(2) Constituents of volatile oil from Yupingfeng compound and Atractylodismacrocephala were analyzed and identified by capillary GC-MS techniques. The resultsshowed that the volatile oil from Yupingfeng mainly contained sesquitrepenes and theirderivatives, The components including seyshellene andα-selinene had high content in volatileoil from Atractylodis macrocephala but were not discovered in volatile oil from Yupingfeng.The composition of volatile oil from compound was not the simple addition of those in singledrugs.(3) On the aim of extraction yield and content of astragalosideⅣ, the extractioncondition of astragalosideⅣextraction in Astragalus membranaceus with ethanol wasoptimized. The optimum condition was 4h of reflux at 80℃with 15 times medicine amountof 55％ethanol. With the aim of extraction yield and content of astragalosideⅣandchromone glycoside, the extraction condition of effective ingredients group extraction in oneautoclave was optimized according to the orthogonal design and single-factor test. Theoptimum condition was 4h of reflux at 70℃with 10 times unit medicine amount of 80％ethanol (4) Active ingredients group of Yupingfeng were separated and enriched with macroporous resin. The adsorption capacity and adsorption ratio of seven kinds of adsorption resinincluding D101 and S-8 for astragaloside and chromone glycoside in extraction of Yupingfengwere investigated. The results showed that the peculiarities of D 101, AB-8 and X-5 were good.Further, the concentration of active ingredients group in solution, flow rate, composition andflow rate of elution reagent affected of leek curves and elution curves were studied. Theresults showed that the purity of astragaloside was raised to 39.27％from 2.57％afterenriched with AB-8 resin bed. Recovery is 83.71％. The purity of prim-O-glucosylcimifuginand 4'-O-β-glucosyl-5-Omethylvisamminol were raised to 3.69％from 0.24％, and 6.34％from 0.31％respectively. Recovery is 82.31％and 91.03％respectively. The active ingredientsgroup was enriched effectively.