Analysis And Nutritional Evaluation Of Components In Grape Seed Ultrafine-powder

Grape seeds are the outgrowth in the field of wine production, and they have affluent nutritional and functional compounds. Exploiting grape seed products, it can not only avoid environment pollution, but also increase wine industry economy benefits. Diameter of grape seed ultrafine-powder is under 25μm, and the cell wall breaking up rate is over 99 %. So grape seed ultrafine-powder can be absorbed more easily, and protect health more effectively. In the experiment, main and functional component contents were detected, and functions of active components were evaluated by analysis of papers.Results were as follows:1 Grape seed ultrafine-powder was abundent of protein, fat, amino acids, inorganic elements, vitamines and so on.2 There were many functional components in grape seeds, including polyphenal, procyanidins, resveratrol, fiber, linoleic acid, vitamine E and so on, and their contents were high, polyphenol 14.22 g/100 g, procyanidins 9.56 g/100 g, resveratrol 8.48μg/g, linoleic acid 81.80 g/100 g grape seed oil, and fiber 39.80 g/100 g.3 Grape seeds of two white varieties (Chardonnay and Italian Riesling) and two red varieties (Pinot Noir and 8804) were powered as superfines by ultrafine comminution. Isoflavones were determined by high-performance liquid chromatography (HPLC) with intern standards to quantify concentrations of individual compounds. Two types of isoflavones, daidzin and genistin were found in grape seeds of all of the varieties detected. But the other two isoflavones (genistein and daidzein) were not found. The total concentration of isoflavones was significantly higher in the red varieties ( 172.962±2.181μg/g for Pinot Noir and 129.966±1.969μg/g for 8804 ) than that in the white varieties ( 49.412±1.601μg/g for Chardonnay and 59.796±1.470μg/g for Italian Riesling), and there was significant differences among the varieties. The main isoflavones in grape seeds seemed to be daidzin ( 156.677±1.859μg/g for Pinot Noir, 120.326±1.392μg/g for 8804, 57.055±1.038μg/g for Italian Riesling and 47.656±1.131μg/g for Chardonnay). These results identified the existence of isoflavones in grape seeds and this may be the first report of isoflavones discovered in grape seeds. This discovery may provide more evidence for the beneficial effects of grape seeds and wines for human beings.