Studies On Extraction And Isolation Of Chlorogenic Acid From Eucommia Ulmodies Leaves And Synthesis Of Molecular Imprinting Polymer For Chlorogenic Acid And Preliminary Research On The Static Absorption Properties

Chlorogenic acid has dramatic effects of heat-clearing and detoxifying, antibiosis and anti-inflammatory, and anti-aging, it has obvious inhibiting effects on digestive tract cancer, also has anti- and adjustment on immune system.This paper laid emphases on the study of extraction, separation and purification of chlorogenic acid from eucommia ulmodies leaves, and the detection of the products. The results are following.1. The extraction of chlorogenic acidFirst, the effects of factors such as solvent, tempreture, time, the radio of material to solvent, times of extraction on the extracion of chlorogenic acid were investigated, respectively, by single-factor experiments, and then by uniform design experiments. The obtained optimal process condition were, 50% of ethanol, 1/30 of the radio of material to solvent, extracting for 50min in ultrasonic bath of 40Hz frequency at 40℃, 4 times. The stability and reliability of the proposed process conditions were tested. The yield of ethonal extract of Eucommia Ulmodies Leaves was 6.38%.2. Isolation, purification and detection of chlorogenic acidUsing lead precipitation as a method of separation of chlorogenic acid from eucommia ulmodies leaves. The crystalline morphology of the precipite was similar to that of chlorogenic acid standard, and the melting-point (208-209℃) agreed with the literature.Alternatively, twice preparative TLC were applied to isolate and purify the ethonal extract. The first preparative TLC using a development solution of methanol-acetic acid (4:0.2) and a silica gel G plate. It was found that chlorogenic acid was seperated from yellow substances which were hard to separate. The second preparative TLC using a mixture of the supernatant of butyl-methanol-water solution(7:2.5:2.5) and a silica gel H plate.The finall products of purifyication were analyzed by analytical TLC using the same condtions sa in the second preparative TLC. The ultraviolet spectrum of the product was similar to that given in literatures. The HPLC main peak and the TLC main spot of the procduct were in accord with that of chlorogenic acid standard. The purity of chlorogenic acid was larger than 91.6% by using HPLC external reference methods. The proposed method is simple, and suitable for commen laboratory to preparate several hundreds milligram to one gramme of chlorogenic acid.The third method of isolation and purification was solvent extraction-precipitation Process. Needle-like sediment was obtained by further TLC separation, it's ultraviolet spectrum was similar to that of chlorogenic acid standard.White power molecular imprinting polymer of chlorogenic acid was made by precipitation polymerization, under the condition of the ratio of EDMA to AIBN was 77mg : 0.97mg. The static adsorption experiment shown that the adsorptions of chlorogenic acid to the imprinting polymer were just little higher than those to the blank polymer in different concentration solutions of acetonitrile, and that water was better than aqueous solutions of acetonitrile for the adsorption of chlorogenic acid.