Study On Preparation Of Microcystin-LR Molecularly Imprinted Polymer Microspheres And Their Enrichment And Separation Performances

Molecular imprinting has been a potential technology which can synthesizethe polymer microspheres that have high recognizable and selective properties toobjective molecules. Over the past thirty years of development, they have beenapplied widely in substantial separation, purification and so on.Using objective molecules as template, molecularly imprinted polymermicrospheres(MIPMs) were prepared successfully by a technology of moleculerecognition in nature. The MIPMs were used to enrich and separate microcystin-LR,thus the aim of this thesis is to treatment organic pollutants, and to provide a feasiblemethod for wastewater treatment in the aspect of inland water.Monodisperse polystyrene(PSt) were prepared by the technique of dispersioncopolymerization. Then using PSt microspheres as seeds, adopting appropriateswelling and polymerization technique, MIPMs which were provided specificadsorption with microcystin-LR were prepared. The factors impacting particle sizeof PSt microspheres were discussed. On the basis of these, monodisperse PStmicrospheres with their particle size between 2.20 and 2.30μm were synthesized byformulas for the optimization of the concentration of styrene, initiator reagent,stabilizer reagent, reaction temperature. In addition, MIPMs with regular spheroidand evenly distributed of particle size were characterized by scanning electronmicrosocope, porosity analyzer, infrared spectroscopy, Thermogravimetric analyzer.The properties of MIPMs with molecule recognition and specific adsorptionwere analyzed. It was showed that the products prepared had excellent adsorptionperformance via proper concentration of imprinting molecules and appropriate usageof methacrylic acid in place of acrylamide as functional monomer. The crosslinkerhad an impact on shape of MIPMs, and the more the amount of crosslinker, the lessthe adsorption amount of MIPMs. The application for enrichment and separation was discussed by the selective experiment. The result showed that the adsorptioncapacity of MIPMs was 39.50μmol/g better than that of its isomer, it also proved thatMIPMs exhibited specific adsorption to microcystin-LR.