Extraction Of Artemisia Capillaries Thunb Flavones And Preparation Of Its β-cyclodextrin Inclusion Compound

Flavone was extracted from Artemisia Capillaries Thunb by five kinds methods, and alkaline cosolvent was used firstly in supercritical CO₂ extraction. The extracting conditions were optimized using the extracting rate of total flavonoids as indicator. Results show that the extracting rate by supercritical CO₂ extraction, sorbitic-extracting-reflux-ethanol method, microwave-assisted-water method, microwave-assisted-ethanol method and microwave-assisted-β-cyclodextrin-solution method under the optimum conditions is 3.88%, 3.08%, 2.90%, 3.09% and 3.68%, respectively. Compared with four others, supercritical CO₂ extraction is a very good technology for extracting flavone from Artemisia Capillaries Thunb. Meanwhile, flavone-extracting rate from Artemisia Capillaries Thunb is remarkably increased byβ-cyclodextrin.Static absorption experiment of macroreticular resin for flavonoids in Artemisia Capillaries Thunb shows that middle polarities macroreticular resin (DM-301) has high absorption ability to flavonoid compounds and 70% alcohol-water solution (v/v) delivers a high desorption effect for the flavonoids in the Artemisia Capillaries Thunb.Inclusion compound ofβ-cyclodextrin and flavone in Artemisia Capillaries Thunb was prepared by coprecipitation method, and its stability, non-oxidizability and bacteriostasis were experimentally tested. Results show that the inclusion effect is the best and inclusion yield reaches 75.5% withβ-cyclodextrin of 1.000g, solvent dosage of 20ml, virgin flavone powder of 1.500g at 25℃for 2h. The solubility of inclusion is 0.032g/mL at 25℃. According to rutin standard, when molar ratio of flavone toβ-cyclodextrin is 1:2 in inclusion compound, the stability of inclusion is greatly increased for ultrasonic and oxygen in comparison with flavone in Artemisia Capillaries Thunb. The virgin flavone powder in Artemisia Capillaries Thunb and the inclusion have evidently non-oxidizability and the former is better than the latter. Bacteriostasis tests indicate that the virgin flavone powder in Artemisia Capillaries Thunb and inclusion have evidently bacteriostasis for Escherichia coli, golden staphylococci and sarcina lutea and the latter is better than the former.