Study On Chemical Modification Of Cellulase And Its Application On Extraction Of Diosgenin

Along with the petrochemical resources and the energy crisis, environment and the society crisis becomes worse increasingly, it is important to change the traditional chemical manufacturing which based on chemical process to the recycled, less- polluted, efficient biological process. The biocatalysis has become one of the important methods for develpoment of the green chemistry and green chemical engineering, and its development will have significant effect on medicine, agriculture, food, life science and so on.For the reason that natural enzymes have properties of protein, natural enzymes are easy to deactivate and the efficiency of biocatalysis will be reduced consequently under the effect of reaction conditions. Cellulase, which is used widely in the extraction and transformation of useful component from natural plants, become instable under the action of heat and the adsorption and desorption between cellulase and substrates. Therefore it is very important to find efficient methods to improve the stability of natural cellulase.Cyanuric chloride was applied to active polyethylene glycol (PEG) and monomethoxy polyethylene glycol (MPEG). Modified cellulases with different kinds and molecular weight were synthesized by activated PEG, activated MPEG and natural cellulase. Experimental data show that PEG-cellulase, MPEG-cellulase and natural cellulase have Michaelis-Menten characteristic, and the order of kinetics constant Km is PEG-cellulase