Studies On The Solid Lipid Nanoparticles Loaded With Pueraria Total Flavones

Pueraria total flavones(PTF)are main effective parts in puerariae, which have showed good effects in the treament of cardiovascular disease. However, its low soluble and low bioavailability limited its clinical application.Pueraria total flavones solid lipid nanoparticles(PTF-SLN) suspension were prepared by emulsification evaporation-solidifying method. According to the experiment results, ratio of drug to lipid, poloxamer 188 and lecithin amount were main influence factors to the encapsulation efficiency. Central composite design-response surface method were used to obtain the optimum prescription. The results showed that the nanoparticles were spherical particles, the encapsulation efficiency was (67.53±0.12)%, mean diameter was (263.82±3.6) nm, and drug loaded capacity was 0.67%. The results of released in vitro showed tally with Higuchi equation. X-ray diffraction experiment showed that the new phase formation has been formed.An HPLC method was developed for the detemination of pueraria total flavones in mice plasma. Oral pharmacokinetic behaviors of PTF suspension and PTF-SLN were investigated in rats. The results showed that oral absorption has a markly improvement in PTF-SLN groups. The AUC0-∞of PTF-SLN is about 2.73-fold higher than that of PTF suspension, Cmax of PTF-SLN is 3.82-fold greater than that of PTF suspension, tmax of PTF-SLN is 1.5-fold later than that of PTF suspension. The results showed PTF-SLN may significantly enhancend the oral absorption and bioavailbility.