| The thesis consists of two parts:review and research reports.Part I:ReviewIn this part, the chemluminescence systems including luminol system, acidic potassium permanganate system, Ce (IV) system, lucigenin system, etc. have been reviewed, based on over one hundred references from 2006 to now, and the mechanisms and applications in food analysis and environmental analysis of luminol chemluminescence systems have been summarized.Part II:Research reportsIn this part, cephalosporins and Sudan dyes were determined, using luminol-dissolved oxygen and luminol-lysozyme flow injection chemiluminescent system.1. A study of the chemiluminescence behavior of cephalosporins with luminol and its analytical applicationThe chemiluminescence intensity of luminol-dissolved oxygen was decreased when cephalosporins were mixed with luminol. The decrease chemiluminescence intensity was linear with the logarithm of cephalosporins concentration over the range from nanogram to microgramme level, with the limits of detection at nanogram level. The sensitivities of determination for cephalosporins were in the order of cefoperazone> ceftriaxone> cefuroxime> cefaclor> cefalexin> cefradine. The proposed method was applied to monitor the excretion of cefradine in human urine after taken cefradine capsules. The possible chemiluminescence mechanism and relationship between the determination sensitivities and generations of cephalosporins were also discussed. This work has been published in DrugMetabolism Letters,2009,3(3):144-151.2. Determination of Sudan IV in hot chilli powder with luminol/dissolved oxygen chemiluminescence systemA sensitive procedure, based on the enhancive effect of Sudan IV on the chemiluminescence reaction between luminol and dissolved oxygen, was proposed for the determination of Sudan IV in a flow injection system. The increment of the chemiluminescence intensity was proportional to the concentration of Sudan IV, giving a calibration graph linear over the concentration from 0.7 ng ml-1 to 300.0 ng mL-1 (R2= 0.9982) with the detection limit of 0.2 ng mL'1 (3σ). At a flow rate of 2.0 mL min-1, a complete determination of Sudan IV, including sampling and washing, could be accomplished in 40 s with the relative standard deviations of less than 5.0%(n=7). The proposed method was successfully applied to the determination of Sudan IV in contaminated hot chilli powder with the recoveries from 89.3% to 108.4%. This work has been published in Journal of the Science of Food and Agriculture,2010,90(2):338-342.3. Flow injection chemiluminescence determination of Sudan IA new method, based on the enhancive effect of Sudan I on the chemiluminescence reaction between luminol and lysozyme in a flow injection system, was proposed for determination of Sudan I. The increment of the chemiluminescence intensity was proportional to the concentration of Sudan I over the range of 7-3000 pg mL-1 (R2=0.9990) with the detection limit of 2 pg mL-1 (3σ). The relative standard deviations were less than 3.0%(n=5). The proposed method was applied successfully to the determination of Sudan I in contaminated hot chilli source with the recoveries of 91.1%-105.4%. This work has been published in Chinese Journal of Analytical Chemistry,2009,37(A01):209-209.
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