| This paper was studied on using fermentation, macroporous resins and ion exchange resins to extract soybean oligosaccharides detected by HPLC from soy molasses.There are 66.58% soluble solid, 8.14% protein, 5.54% ash content and 48.61% total sugar which contain sucrose 317.53mg mL-1, raffinose 43.66mg mL-1 and stachyose 175.42mg mL-1.Screening of yeasts, lactobacillus, Monascus rubber and mixed culture fermentation of them, 7 yeast is selected which can decompose sucrose as well as retain raffinose and stachyose, raise functionality of oligosaccharides from 40.24 % to 88.57% in total sugar.The results of odd fator tests 7 yeast showed that the optimum fermentation conditions for soybean molasses was: the inoculation concentration was 2%, the temperature was 28℃, the initial pH was 5.0, the soybean oligosaccharide dilute 8 times,after 12 h the remainder rate of residule sucrose 5.76%, raffinose 99.61%, stachyose 95.72% in the fermentation broth of soybean molasses.Evaluating whether fixed pH and dissolved oxygen content to ferment by fermenter of volum 20L, we defined the optimization condition is not necessary to control pH and oxygen content in the course of fermentation, at this time, the remainder rate of residule sucrose 19.69%, raffinose 98.07%, stachyose 99.96%.The optimum conditions of Acid-Precipitatio protein was: 4% HCL, pH 3.0, stir 10min, centrifuge 10min. The result of perpendicular is deposit protein rate 55.50%, total sugar remainder rate 99.70%, functionality of oligosaccharides raise from 53.30% to 58.65 in soluble solid.We selected three macroporous resins to decoloratio the fermentation solution after Acid-Precipitatio and definited AB-8 is best for decoloratio. The optimization condition is taht the flow rate is 1.5BV/h, the volum of decoloration is 4.5BV, decoloratio rate is 92%, total sugar remainder rate is 99.70%. The total sugar occupied 75.40% of soluble solid. The content of sucrose is 2.23mg mL-1, raffinose is 5.31mg mL-1 and stachyose is 20.51mg mL-1, the protein is 0.14mg mL-1.001×7 cation exchange resin combined 201×7 anion exchange resin or D301 anion exchange resin to ion exchange the crude soybean oligosaccharides and the result is 001×7 combining D301 is better. The optimum conditions is that the ion exchange resins combined according to the cation antecedently, ordinary temperature, flow rate is 1.2BV/h and under this condition 001×7 exchange capacity is about 9BV, D301 is about 6BV. The result of ion exchange is that the solution of soybean oligosaccharides has no nitrogen-containing material. The total sugar is 89.74% times of soluble solid. The content of sucrose is 2.05mg mL-1, raffinose is 4.88mg mL-1 and stachyose is 18.87mg mL-1, and functionality of oligosaccharides occupied 86.51% of total soluble solid.The freeze-dried solution of soybean oligosaccharides obtained from 001×7 combining D301 is white, taste somewhat sweet, the total sugar content is 0.94 g·g-1 and from 001×7 combining 201×7 is light yellow, and the total sugar content is 0.83 g.g-1.
|
PDF Full Text Request