Research On The Phosphatidycholine Enrichment And Removal Of Pyrogen

Phospholipids is a mixture of phosphorus lipid, with different physicochemical and functional characteristics. It has been widely used as an emulsifier in the food and pharmaceuticals industries. The aim of the present work was to establish an optimized route for phosphatidycholine enrichment by the ethanol heating extraction and frozen purification method, and removal of pyrogen by active carbon technologies. The new products can be injected were produced.The soybean lecithin products with PC content in 72-85% and PE content in 5-10% were extracted from soybean powder phospholipids. The effects of ethanol concentration, ethanol/lecithin ratio, extraction temperature, extraction time, freezing temperature and freezing time on product yield and the PC and PE content were investigated. High yield was expected, as well as the PC content and PE content of the product ranged from 72% to 85% and from 5% to 10%, respectively. The results showed that the superior conditions were 95% ethanol concentration, ethanol/lecithin ratio 4:1, extraction temperature 60℃, extraction time 45min, freezing temperature -15℃, freezing time 30min. Based on the results of factorial tests, response surface methodology (RSM) was utilized to further investigate the effects of ethanol/lecithin ratio, extraction temperature, freezing temperature and freezing time. Under the optimized conditions, i.e. ethanol/lecithin ratio 4.50:1, extraction temperature 62.75℃, freezing temperature -13.96℃, freezing time 30.00min, the product yield was 24.17%, PC content was 73.89%, PE content was 10.00%.The feasibility of the bacterial endotoxin test by Tachypleus Amebocyte Lysate(TAL) for soybean lecithin products was studied. The interfering effect was not shown when the soybean lecithin solution with the concentration of 10mg/mL was diluted to 3 folds. TAL can determine the number of units of endotoxin in phospholipids. Using limulus bacterial endotoxin test to detect the endotoxin level of injectable soybean lecithin is feasible.The activated carbon was used to adsorb the pyrogen from soybean lecithin. The effects of the dosage of activated carbon, adsorption temperature, adsorption time on removal effect were investigated. The minimum concentration of endotoxin was expected. The results showed that the superior conditions were the dosage of activated carbon 0.5%, adsorption temperature 50℃, and adsorption time 40min. Based on the results of factorial tests, the orthogonal experiment was utilized to further investigate the effects of the dosage of activated carbon, adsorption temperature, and adsorption time. Under the optimized conditions, i.e. the dosage of activated carbon 0.6%, adsorption temperature 50℃, and adsorption time 40min, the concentration of bacterial endotoxin of soybean lecithin was 4.41EU/g, the product yield was 89.65%.Emulsibility of the injectable soybean lecithin was also studied. Using the stability index of centrifugation as the standard, the fat emulsion prepared by the soybean lecithin with the PC content in 70-85% showed good emulsion stability. The scope of HLB (hydrophilic-lipophilic balance) number in about 7.5-9.5.