| Objective:to screen the extraction technology,the filming formula and the plastic packaging material of ShuangBai Consperg Film Agent;to investigate the solution capability of the various pellicular materials into the sample solution; to investigate the dissolution and the filming Performance of the pellicular materials in the extraction Solution of CM by using different concentration of the ethanol.Methods:(1) Combining the pharmacological experiments and the content detection of index components (Aloeemodin,Rhein,Rheum emodin,ChrysopHanol,PHyscion,Berberine,Palmatine chloride,Quercitrin,Caffeic acid), with the ShuangBai Spray as a comparison, and screen the extraction technology of ShuangBai Consperg Film Agent by comparing the process of water extraction and alcohol precipitation,the ethanol refining process and the combined prescription process of four refined parts;(2) Do filming experiments with polyvinylpyrrolidone(PVP),Hydroxypropyl Methyl Cellulose (HPMC),Glycerol,etc, with solubility. Jet Performance,viscosity. Film-forming properties. Film-forming time as indexes, formulate the Film-forming Process Route of ShuangBai Consperg Film Agent; (3) respectively Take the five purificatory materials and Shuang Bai Spray into PET Spraying bottle,PP Spraying bottle,HDPE Spraying bottle, after putting these samples 0 month,1 months,2 months,3 months,4 months,5 months,6 months, and then sample for checking Characters,determining content and observing the Color changes of the Spraying bottles, screening the best suitable Packaging material of ShuangBai Consperg Film Agent by analyzing the mutual influence situation between Spraying bottles and samples of the different Chinese Medicine; (4) prepare different sample solutions which contain water,20%(V/V) ethanol solution,40%(V/V) ethanol solution,60%(V/V) ethanol solution,80%(V/V) ethanol solution and 100% (V/V) ethanol solution, and then investigate the solution capability of the various pellicular materials into the sample solution; (5) according to the results of the dissolving experiments of the pellicular materials, select PVP,PVA,Ethyl cellulose (EC),Methyl cellulose (MC),Polyvinyl butyral (PVB) etc, and investigate their Dissolved situation and Film-forming properties in the different ethanol extracting solution of the different Chinese Medicine (Radix et Rhizoma Rheiand,Cacumen Platycladi,Amur CorKtree BarK,Lycopus,mint) by analyzing the filming photos which were screened by the microscope and photoing software.Results:(1) the index components of the combined prescription process of four refined parts were higher than the process of water extraction and alcohol precipitation, but the combined prescription process was very complex. the process which contained ethanol refining,concentrating and adjusting 40%(V/V)ethanol was better than the process of water extraction and alcohol precipitation in terms of the contents analysis and pharmacological experiments, moreover in condition of saving 80% medicinal material; (2) using 5% PVP-K30 and 0.8% HPMC would quickly form a layer of uniform, density film in the ethanol extraction solution of Shuang Bai prescription;(3) HDPE spraying bottles which contained shuangbai spray and total Refined anthraquinone parts of Radix et Rhizoma Rheiand had Obvious color changes. In addition, the Concentration of chrysopHanol and pHyscion in the HDPE spraying bottles appeared a obvious declining tendency but the changes of the Concentration and color of the PET spraying bottles and PP spraying bottles were not obvious; (4) PVP would quickly dissolve in any concentration of ethanol solution;the Solubility and film-forming Properties of PVA became worse and worse as the the concentration of ethanol solution increased; MC were extremely thick when they were dissolved, but the dissolved solution was very muddy in the water and 20%(V/V),40%(V/V) ethanol solution, and the Solubility associated with the concentration of ethanol solution; EC and Acrylic resin would dissolve in the 60%(V/V) ethanol solution at least; PVB would dissolve when the concentration of ethanol solution was very high;(5) the film properties of PVP-K30 and PVP-K90 in all kinds of the extraction solution of Chinese Medicine were very fine, and the texture of the membranes were very dense and smooth, but the film properties of PVP-K90 in the extraction solution of the 40%(V/V)ethanol solution and 60%(V/V)ethanol solution were fairly poor; PVA in the extraction solution would form membrane structures, but the surface of the membrane was uneven and rough;the Solubility and Film-forming Properties of Ethyl cellulose(EC)in the extraction solution of 100%(V/V)ethanol solution of CM were very fine, and EC would form a thick,smooth and dense film, but the Solubility and Film-forming Properties of Ethyl cellulose (EC) in the extraction solution of 80% (V/V) ethanol solution of CM were poor;the film properties of MC in all kinds of the extraction solution of Cacumen Platycladi and Amur Corktree Bark were very well and MC would form a thick,smooth and dense film, but the film properties of Methyl cellulose(MC) in other extraction solution of CM became worse and worse as the the concentration of ethanol solution increased; PVB in the extraction solution of 100%(V/V)ethanol solution of CM would form a film, the film properties specially in the Cacumen Platycladi and Amur Corktree BarK were very well.Conclusion:(1) the extraction technology of 75%(V/V) ethanol extracting,concentrating and adjusting 40%(V/V) ethanol concentration can be used as preparation process of ShuangBai Consperg Film Agent, and the process provide a research foundation for futher studying; (2) the filming formulation of 5% PVP-K30 and 0.8% HPMC would have a excellent film, and PVP-K30 and HPMC can be used in Consperg Film Agent of CM;(3) the PET spraying bottle is the best Packaging material of the Shuang Bai Consperg Film Agent, and the results thow that different plastic bottle have different interaction in the extracted solution of CM, and selecting the plastic Packaging material should consider this condition; (4) studying the ethanol dissolving range can provide reference for using film material:PVP can quickly dissolve in any concentration of ethanol solution;the Solubility and Film-forming Properties of MC and PVA become worse and worse as the concentration of ethanol solution increase; Acrylic resin,PVB,EC and can dissolve when the concentration of ethanol solution is high;(5) investigating the film-forming properties of the film material in the different ethanol extracting solution of Chinese Medicine can provide reference for the Consperg Film Agent of CM:PVP can quickly dissolve in any concentration of ethanol solution, and their film-forming Properties are very fine; PVA in the extraction solution of CM would form membrane structures, but the surface of the membrane is rough;the film properties of MC in all kinds of the extraction solution of Cacumen Platycladi and Amur Corktree BarK were very well, but the film properties of MC in other extraction solution of CM become worse and worse as the the concentration of ethanol solution increased;the Solubility and Film-forming Properties of EC and PVB in the extraction solution of 100%(V/V)ethanol solution of CM are very fine, and PVB in the extraction solution of 100%(V/V)ethanol solution of Cacumen Platycladi and Amur Cork tree Bark are very well.
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