| The information coming from microcirculation is idiographic, profuse and one of the indispensable parts to a integrant life-form . It is also an inimitable way for the corporeal exchange between living cells and external milieu. Based on the study, the change of vital sign(VS) will influence the functions of any other organs . There will be a severe influence to the parafunctional organs . The researcher has paid their consanguineous attention to the microcirculation's change caused by the VS's change. So, the synchronized detection and observation between the microcirculation and VS is very important. The objective of this subject is to develop such a software system that can get the values of VS during the process of microcirculatory detecting experiment. So now in the world, there is not any reports about this.Based on the research of microcirculatory experiment's theory and technique, a system using a programmable language LabVIEW is designed with all-purpose- baroceptor,temperature detector,DAQ card,digital camera,PC and IMAQ card. This system makes microcirculatory experiment's image collection and deposition more precise and reliable with new functions of detecting VS in real time. The system can detect the VS(BT,BP,HR and MABP etc.) with detecting,measuring and recording the microcirculatory parameter .Also the detected data can be analyzed,saved and printed with this software system .With the change of detecting laser head or the appointed microcirculation microscope we can detect the different part of body's microcirculation with preferable dependability , such as detecting the microcirculation of bulbar conjunctiva,eyeground or mesenterium and so on. Flexibility and expanbility, the common characters in VI, is the reason that the system can be used in other fields by adding new module and expanding functions.MethodsLabVIEW programmable language is the primary program language in this system. Image collection function module and data collection function module are the primary function modules of this system. At the beginning set the parameters of image collection, including the luminance and field of vision. Then test the real distance between one pixel and the next one in the CCD using a standard module. Run the main program, save the images detected by CCD as the named AVI file, in the same time the system is processing every frame image in real time. Based the blood cells shape and its mean pixel , the system recognises the blood cells from surroundings as the processed objects. When the the blood cells are recognised by the system , they will be numbered , and the number is from one to n. The system default the number zero as the studied object. Also it can detect the number of the blood cells in the real time, for the blood flow will make the blood cell flow out of the field of view, when the number zero flows out of view, the system will remark the recognised cells and make the new number zero as the studied object. When the studied object changed bucause of the hemokinesis , it will cause an error. And for the purpose of avoiding the error we designed a sequence to ensure the change of studied object can't cause the analytic data mistaked . With some disposal , system will give us the results of detecting data that is analysed as the result of collection time,blood flow rate(BFR) and the state of microcirculatory blood stream in real time. The system can process the saved data in the end of the experiment and recur the course of the experiment by playing the saved AVI files. The system will begin to detect the VS and display the VS data in real time when it detects the order to detect the VS from user. Mutative tendedcy can be observed from the control of Waveform Chart in the frontal panel. Users can choose the appropriate time to save the valuable data , also we can detect save and dispose the data in real time, recur the correlative data in the end of the experiment.ResultA multifunctional detection System programmed by Virtual Instrument LabVIEW can detect the VS of intravital experimental animal during the progress of the microcirculatory experiment .A new algorithm in the field of microcirculatory experiment is advanced that it can compute the blood flow rate without tracking the specified blood cell . The change of the studied object's number can't influence the run of the software and the dispose of the data. The system make the real-time-detect,data-analysis and data-save came true and collected data is accurate. AVI file and LVM file is the two forms to save the experimental result. The AVI file records the whole process of experiment which can be replayed by AVI player and analyzed by other software. The LVM file saves the blood flow rate data and the VS data of the distinguished object which is saved as a pair of number and will be read to replay the blood flow rate and the changed tendency of VS.ConclusionsThe system based on LabVIEW for microcirculatory experiment's image collection can record,save and analyze the VS data obtained from experiment with more functions than any other image collection systems. The software system has a preferable stability and the experimental results show a better veracity and sensitivity by the intravital rat's experiment. The system offers a new method for the experiment that can detect the VS during the course of microcirculatory experiment, it also offers a new approach to study the quantitative relationship between them.
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