Culture Of Dendrobium Candidum And Tissue Culture

Many problems exist in Dendrobium officinale tissue culture production, such as long period, turn bottle more often and complicated operation. In this paper, cultivating the plantlets and proliferating clustered buds were studied. In order to reduce the steps of cultivating the plantlets and clustered buds, shorten the growth time and simplify the experiment operation, single-factor and multi-factor orthogonal experiments were carried out to study the influence of basal medium, plant growth regulators, natural addition, time and other factors on the cultivation of the plantlets, induction of axillary buds and proliferation of clustered buds. The results showed that:The study of the plantlets cultivated from mature seeds showed that:the optimal medium for seeds germination was N6+6-BA 0.1 mg/L+NAA 0.5 mg/L+potato 100g/L +active carbon 2 g/L. Then the best plantlet strengthening medium was N6+6-BA 0.1 mg /L+NAA 0.5 mg/L+potato 100g/L+active carbon 2 g/L. Another study of the plantlets showed that:1/2 MS is more suitable to cultivate culture seedlings by using mature seeds as explants. The optimal medium for protocorm germination was 1/2MS+taro 100 g/L. Then the optimal medium for protocorm multiplication and differentiation was 1/2MS+ potato 100 g/L+banana juice 100 g/L+6-BA 0.2 mg/L. And the best medium for strengthening and rooting was 1/2MS+potato 100 g/L+banana juice 100 g/L+NAA 0.5mg/L. These two methods are feasible, short in time, simple in operation and the plantlets were green and strong.After axillary buds induction, clustered buds were proliferated from stem segments. Results showed that potato was conductive to induce axillary buds, the optimal medium for axillary buds induction was N6+6-BA 1.0 mg/L+NAA 0.2 mg/L+potato 100g/L. And banana was benefit to proliferate clustered buds, the optimal medium for clustered buds proliferation was N6+TDZ 0.05 mg/L+6-BA 1.0mg/L+NAA 1.0 mg/L+banana juice 100g/L. The time was better to be 60 days in proliferating clusters from stem segments. The clusters proliferated of 60 days were strong and can be able to strengthening and rooting culture.The study of clustered buds proliferated from aseptic seedlings showed that:Banana was conductive to proliferate clustered buds, the optimal medium for clustered buds proliferation was N6+TDZ 0.05 mg/L+6-BA 1.0mg/L+NAA 0.6 mg/L+banana juice 100g/L, and the proliferating time was also better to be 60 days. Compared to aseptic seedlings, stem segments had higher multiplication coefficient of clusters. And clusters proliferated from stem segments were stronger than those proliferated from aseptic seedlings. However, proliferating clusters from aseptic seedlings is short in time and simple in operation, since without induction of axillary buds. Clustered buds proliferated from stem segments and aseptic seedlings can be able to strengthening and rooting culture. The growing conditions and processes were optimized to improve multiplication coefficient and shorten the growing time of clusters. In order to solve the problem that Dendrobium officinale resources are scarce, feasible systems of proliferating clustered buds were established to provide valuable technical information and scientific bases for rapid seedling and factory production.(All culture medium added sugar 30 g/L, agar 6.0 g/L, pH 5.8, the medium that containing banana juice added agar 7.0 g/L)...