Primary Study On The Doubling Methids And Fertility-Restoring Mechanism Of Maize Haploid | Posted on:2002-05-08 | Degree:Master | Type:Thesis | Country:China | Candidate:J J Wei | Full Text:PDF | GTID:2133360032451299 | Subject:Crop Genetics and Breeding | Abstract/Summary: | PDF Full Text Request | Using parthenMentlc Inducing line s polllna比Cthe stUdies on the Inducing haploids from different genotype female materials was carried ow.The n扯wal fertility ofear and inflorescence of the Induced hå©fold was observed.Some haPlold p枯ms were treated sep盯ately with different colchiclne concemration nd 2ï¼…DMSO in six leaf nd elongation stage to double their chfomosomes.At the same time flowering condition of the male Inflorescence was observed.The result硼some haplolds had nthers but c。uld n。1Shed,S。p。11en Sï¼»I侧fo1es8wem mMe 10 these hs帅The æ–¹rtllityï¼restoring mechanism of the haplold was analyzed using cytology method.The pollen sheddinghaploid plants were selFpollinated to getdouble haploid(DH)lines. Agronomlc traits was observed,and polyacrylamlde gel electrophoresis(队GE)of Peroxldase(POD)and Esterase(EST) lsompe was done to DH lines. Although90.52ï¼…ofhaploidears were fertile,the fertileness ofthetassel were ic风 and pollen sheddlæ— ability wasdifferent betweengenotypes.The lowest shedding rate was 3.03ï¼…,while the hehest reacheu as hç‚’ as 50.00%.So the ability of the haploid seedï¼setting by scåœpollination was resmctCd by the feftileness of眺咒l.yor haplold chromosome doubling treatments with different colchicine nd 2.0ï¼…DMSo,the best result Is the treatment which was made In six ie对stæ˜e with 0.5ï¼…colchlclnes concentration.It was be地r than 0.1ï¼…concentration.In elongation stæ˜e the rate of drug lm"red plants was high,and the change of*the haploid doubling rate was little.gll of*the tested haplolds have stalnable pollen.Theå«erage frequency Is 8.0 po比ent.The highest Is 20.0 percent.The study also found that dlploid cell exited both In somatic cells and In pollen mother cells.Only lfdlplold cell was In somatic cells,It would appear In PMCs. The DH lines showed highly uniformity In all traltsthat can be measured.Some ofthem 41could be accepted for breeding pnå“Dsc ingrowing,ear size nd otherï¼…ronomlc traits.The results of the PAPAGE of POD and EST isowme showed that some bands of the DHlines was the same s the male material and some was the sme s the female material.ItIs therefore concluded that theå‘»henogentlc Inducing line Induced haplold Is a randomprocess....
| Keywords/Search Tags: | maize, haploid, chromosome doubling, fertility-restoring mechanism, DH lines | PDF Full Text Request | Related items |
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