Study On Genetic Diversity Of Tibetan Mastiff Based On The Blood Protein Polymorphisms

Study on Genetic Diversity of Tibetan Mastiff Based on the Blood Protein PolymorphismsPresented by Lan XiaopingFaculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070Supervised byAssociate Prof. Cui TaibaoFaculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070A total of 103 blood samples was taken from four populations of Hequ Tibetan Mastiff, Qinghai Tibetan Mastiff, Qinghai Tibetan KyiApso and native dogs of Qinghai and analyzed on the blood protein polymorphisms by vertical polyacrylamide gel electrophoresis (PAGE). Genetic variations were observed in Tf, S a ,, Po, Es-1 and Es-2. There were three alleles at the loci of Tf and Po, two alleles at the loci of S a 2 and Es-2, genetic variations of Es-1 is controlled by four alleles. The degree of genetic variability estimated by Nei's expected average heterozygosity for the 9 blood protein loci was relatively higher in Tibetan Mastiff (0.2723) than that in native dogs (0.2160). The average genetic diversities within Tibetan Mastiff populations (//$) and in the total Tibetan Mastiff (Hj) population are 27.22% and 27.72%, respectively. The average genetic diversity between Tibetan Mastiff populations (Z>sr) is 0.0052. The genetic differentiation of total Tibetan Mastiff population (GST) is 0.0187, which indicated that there is less variation (1.87%) among Tibetan Mastiff populations and the genetic differentiation is very limited between Tibetan Mastiff populations. Furthermore, A dendrogram was constructed by unweighted pair-group method with arithmetic mean(UPGMA)based on Eucledean genetic distances among the populations. It is suggested that the Tibetan Mastiff descended from different origins of dogs from other Chinese breeds, Japanese dog breeds, Spanish dog breeds, Korean Jindo and Beagles.