| This paper is about physiological and biochemical analysis for bulbs of lily (L longiflorum) cv. 'Snow Quuen1 which grown in Hangzhou city. The concentrations of total sugar, starch, reducing sugar, sucrose in the scales of lily were analyzed for dissimilation and assimilation of carbohydrates. Leaf photosynthesis, protein and activities of some enzymes (including superoxide dismutase, catalase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase) in bulbs were also dertermined during the plant growth period and the cooling storage of bulbs. In addition, the proteins contents in bulbs and leaves of lily in different developmental periods were discussed using the method of SDS-PAGE gel electrophoresis. The results are as follows:lit was found that the fresh and dry weight, perimeter of lily bulbs increased significantly after harvest. Disbuttoning treatment influence the bulb development and the fresh weight increased dramatically in the harvested bulbs. Disbuttoning was showed as an effective way of accelerating the bulb development. The plant high is shorter than when flower-bud was removed. The total number of leaves was same with the treatment of disbuttoning, it indicates that there is no influence of photosynthesis in lily plant.2.The total sugar and starch are the main source of carbon nutrient. From planting to bud stage, the concentrations of starch in bulb decreased, and then increased. But the contents of total sugar in bulbs increased remarkedly before bud stage, then decreased rapidly. The concentrations of total sugar and starch in harvested bulb were about 85 mg/gFW, and 200 mg/gFW, respectively. Anaylsis of the changes of starch and total sugar in bulbs, we considered that the nutrients of bulb are outing for the plant growth before the bud stage. The nutrients were accumulated for bulb development when the bud was removed.3. From the photosynthesis experiments, we found that the net photosynthetic rate is 0 when the PFD is 70-80 v- molM^S"1. The top leaves'Pn was 6.15ii molC02M"2S~1 as the saturation level when PFD was 730 u molM"2S"1. Pn in the top leaves showed higher than the other leaves.4.Catalase and peroxidase are involved in reducing the infection of alive oxygen. The activities of catalase, superoxide dismutase and peroxidase showed high when start to growth. Their activitives have a trend of weaken before harverst. The activity of polyphenol oxidase increased from planting to bud stage, and then decreased slihgly. The highest activity of polyphenol oxidase was observed at beginning of growth. The activity of phenylalanine ammonia-lyase increased obviously before bud stage, followed by a decrease until harvest. With these observations, we concluded that the nutrients in bulbs consumed quickly during the plant growth, and the increasing of the biochemical activities as well as the carbonhydrates accumulation in bulbs demonstrated the bulb development. The bud stage could be regarded as the start of bulb regenerated.5. By using the method of SOS-PAGE gel electrophoresis, the total contens of protein in bulbs and leaves in different growth periods were analyzed. Bands of protein in bulbs showed that no difference in various developmental periods, but there were some differences between bulbs and leaves. Analysis of protein bands also indicates that the abundance of proteins exists in lily bulb during the bulb development.6-During the cooling storage, the contents of total sugar in bulbs increased at first month, and then decreased slightly. Concentrations of starch decreased very slowly at the beginning. The total carbohydrates decreased slightly during the whole storge. The activity of CAT and POD and PPO increased at fist month, whereas the CAT activity decreased slowly. The activity of POD and PPO showed a stable level during the storage. Increasing of the activities of related enzymes only at the first month and stable level of carbohydrates in the whole storage maybe reflect the physiological mechanism during the dormant period of lily bulbs.
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