Analysis Of Chinese Merino And Other Three Breeds Of Sheep By RAPD

Random amplified polymorphic DNA (RAPD), as a technique of molecule genetic marker based on PCR, has been extensively used to analyzing genetic diversities, measuring the genetic distance among or within species and marker assistant selection (MAS). In this study, the molecular polymorphisms of Chinese Merino sheep (Xinjiang's), Hasake sheep, Maigaiti sheep and Luomuni sheep (from England) were analyzed by RAPD. Total of 120 primers were used to amplify the pool DNA of four sheep breeds as well as individuals respectively. Among of the 120 primers, 22 primers generated sufficient polymorphisms steadily. The total number of RAPD sites derived from 22 primers was 167, of which 83 were polymorphic, accounted for 49.3﹪. The genetic distances of Chinese Merino sheep (Xinjiang's) between Hasake, Lumuni, and Maigaiti sheep were 0.1707,0.1791 and 0.3087, respectively. According to the cluster analysis chart, the genetic relationship between Chinese Merino sheep (Xinjiang's) and Hasake sheep was the very closed, following is Lumuni sheep. Maigaiti sheep was comparatively further to the other three breeds. This was correspondent to their real physiology feature, genetic relationship, historical origination and geographical distances. Meanwhile, the RAPD analysis was conducted in four lines of Chinese Merino sheep (Xinjiang's)(line of superior wool, line of big body, line of dense wool and line of super-fine wool) with 120 primers. Three random primers were identified with polymorphism. The outcomes of RAPD analysis demonstrated that there was a low level variability among these 4 lines. According to the polymorphism of pool and correspondent individual DNA of four lines generated by the primer of OPF15, a specific fragment was observed only in the line of super-fine wool. The molecule size of the specific product was around 826bp, which was named OPF-15826. It can be served as a marker used for MAS of line of super-fine wool sheep. Moreover, a stable RAPD PCR system was established by optimizing the factors that affect the sensitivity, specificity and stability of PCR.