The Studies On The Detoxification Of Castor Bean Meal By Microorganism

Detoxification of CBM using method of microorganic fermentation was discussed in this paper. In these studies, 2 microbes (DT fungus and JM fungus) were adopted. The time of fermenting CBM by DT and JM funguses involved respectively 3 stages: 5 days, 10 days and 15 days. After the trial of fermentation, the content of ricinine and allergen was detected. Following the trial of fermentation, the trial of feeding white mice with undetoxified and treated CBM was applied. The CBM selected to add into white mice's ration include the samples treated by DT fungus for 10 days^ JM fungus for 5 days and the untreated sample. The adding proportions of each kind of sample were 5%, 10% and 15% respectively. At the end of feeding trial, all white mice were anatomised to detect the remnant ricinine and allergen in the liver, kidney and heart.The results of above trials indicated ricinine content in CBM treated by DT fungus for 10 days and 15 days, and by JM fungus for 5 days and 10 days was eliminated high significantly (p<0.01). Compared with undetoxified CBM, ricinine content in CBM treated by DT fungus for 5 days and JM fungus for 15 day was significantly low(p<0. 05). Tn addition, the difference of allergen content between undetoxified CBM and CBM treated by DT fungus for 5 days and 10 days was significant(p<0. 05) and high significantly (p<0. 01) respectively. Yet in the other groups of treated CBM, there was no allergen can be detected. Feeding trial showed no death of white mice induced by both undetoxified and treated CBM. But ricinine was found in the livers of white mice fed with undetoxified CBM when its propotion was above 10% in diet. There was no remnant of both ricinine and allergen found in the groups of white mice fed CBM treated by JM fungus and DTfungus. Considering such factors as the detoxification efficiency, treating time, influence on general nutrient of CBM and remnant toxin in white mice viscera etc, the conclusion of the studies is: of those deloxifing method adopted Lo CBM, using JM fungus to treat CBM for 5 days is the best one.