Studies On A Comparison Of JUNCAO Shii-take And Sawdust Shii-take Quality And JUNCAO Substrate Screening

Purpose: JUNCAO substrate was screened and optimized, and a comparative studies on yield and quality of JL'NCAO shii-take and sawdust shii-take, so as to scientifically appraise to shii-take cultivated with JUNCAO and offer scientific basis for production and development of JUNCAO shii-take.Method: Use tertiary system method to screen and optimize JLNCAO substrate, and use heat and dry under atmospheric pressure method, ignition residue method, The Casparian law of trace nitrogen, acid detergent law, automatic analysis instrument of amino acid, the fluorescence spectrum of atom and atom absorb the spectrum, acetyl acetone method and hot water extraction polysaccharide method to measure the content of the moisture, ash content protein, cellulose, amino acid, heavy metal, formaldehyde, water soluble polysaccharide of JUNCAO shii-take and sawdust shii-take was comparatively studied separately.Result: The yield of one bag cultivated with JUNCAO was lower 10.77% than that of cultivated with sawdust, but the bioconversion rate was higher 12.08%. The contamination rate of bags filled with JLNCAO was slightly higher than that filled with sawdust. The growth rate of mycelia cultivated with JUNCAO was faster than that cultivated with sawdust.The result of this experiment showed that the content of protein of the JUNCAO shii-take is 1. 98 times than that of sawdust shii-take. The total amino acid content and essential amino acid content of the JUNCAO shii-take was higher than that of sawdust shii-take. But proline didn' t measured in the JUNCAO shii-take.The content of crude cellulose of the JUNCAO shii-take was lowerthan that of the sawdust shii-take. The water content of the JLNCAO shii-take was higher than the sawdust shii-take slightly. Therefore the JLNCAO shii-take was tenderer, but marginal veil was more smashed after dried, if picked too late.The content of some heavy metal and formaldehyde of the JLNCAO shii-take was higher than that of the sawdust shii-take. But its content was under the standard range of the regulation in our country and world, and not been harmful to the human body.Enzymatic solve optimization terms showed that the removal of the proteins with enzyme and Sevag method was superior to with Sevag method independently. Pepsin and trypsin optimum condition were respectively Pepsin enzyme-added about 1.5% of polysaccharide quantity, pH about 2. 0, keep temperature three hours under 37℃ and trypsin enzyme-added about 1%, pH about 8. 5, keep temperature three hours under 37℃.The effective polysacchar ides content of the JL'NCAO shii-take was 3.25 times than that of the sawdust shii-take, they both have obvious antitumor effect. It was 44.93%, 43.95% respectively to restrain the tumor rate.Therefore the shii-take can entirely use JLNCAO as compost. The yield and quality of the shii-take cultivated with JLNCAO were not second to that of the shii-take mushroom cultivated with sawdust. L'nder the timber shortages conditions, the humankind can culture shii-take with JLNCAO instead of the sawdust and cottonseed shell.