Studies On Inheritance And Breeding Application Of Descendants From Anther Culture Of Photoperiod-Thero-Sensitive Genic Male Sterile Rice

In order to find out the possibility of purification and improvement of PGMR through anther culture technique , and provide reference for application of anther culture breeding in two-line hybrid rice , systematic studies on inheritance of pollen fertility and major agronomic traits , combining ability , affinity , and yield in hybrid F1 and traits variation in hybrid F1 of the anther culture descendants from PGMR have been carried out in this paper . The conclusions are as follows :1. There is diversity and relative stability in the fertility of anther culture descendants from PGMR . Different plant lines which come from the same material have different pollen fertility . But in pollen sterile period , the sterile pollen rate of most anther culture descendants from Peiai64S approaches or surpasses to their contrast parents' , furthermore , the variation coefficient of sterile pollen is close or low to the contrasts' . All the sterile pollen rate of the anther culture descendants from PeixuanS exceeds to their contrast PeixuanS' in pollen sterile period , and the variation coefficient of sterile pollen is lower than the contrasts' obviously .2. Fertility in different generation from anther culture descendants of PGMR is stable basically, and it doesn't alter obviously according to the change of temperature in the sensitive period of pollen fertility .3. Fertility in ratooning plants from anther culture descendants of PGMR is relate to that in their seeding plants . If the sterile pollen rate of seeding plants is high ,the sterile pollen rate of ratooning plants is also high, otherwise , the sterile pollenrate of ratooning plants is low .4. The major agronomic traits of the H3 generation pollen plants from PGMRapproach or surpass to their contrast parents'.5. The combining ability in comprehensive traits of the H3 generation pollen plants from PGMR approaches or surpasses to their contrast PGMR , therefore , the plant line which general combining ability is high does not own high special combining ability certainly ; the plant line which general combining ability is low also does not own low special combining ability surely .6. The higher yield two-line hybrid rice combinations will be gained , if we use the descendants from anther culture of PGMR as female than use the contrast PGMR.7. There is the similarity on affinity between anther culture descendants of PGMR and contrast PGMR , if the affinity of contrast PGMR is high , the affinity of its anther culture descendants from PGMR is also high , otherwise the affinity of pollen plants of PGMR is low .8. The major agronomic traits of F2 generation from the pollen plants of PGMR is similar to those from their contrast parents.