Study On The Technique System Of Micropropagation In Rhododendron Argyrophyllum

The shoottips and stems of Rhododendron argyrophyllum were used as explants to establish, a rapid technique system of micropropagation. Root and leaf of Rhododendron argyrophyllum were used to find out the best medium compoisition of callus induction, hi the experiments , effective measures were found out to prevent the browning and contamination of shoot explants .The results were as follows:1) Suitableculture conditions were:Day and night 25℃±1℃,14 hours illumination every day, and about 2000 lux illumination intensity and medium pH 5.3~5.6.2) The best technique for initiation culture was Anderson+6-BA0.1mg/L + NAA0.01mg/L+,for a week of dark treatment. After 4 weeks culture, the rate of initiation could reach 60.7%.3) For multiplication of shoot clumps, Anderson+6-BA1.0 mg/L was the best medium. After 12 weeks culture, the multiplication rate of shoot clumps could reach 4.2.4) For elongation of the shoots, Anderson +6-BA2.0 mg/+NAA0.01 mg/L+AC1.0/Lwas the best medium. After 90 days culture, almost all the shoots' the length could reach 2cm.5) For the rooting of shoots, Anderson +AClg/L was the best medium.6) The best medium composition for callus induction from sterile seedling's leaf explants was Anderson +6-BA1.0 mg/L+2,4-D0.1 mg/L,for 2 weeks of dark treatment.7) The best medium composition for callus induction from sterile seedling's root explant was Anderson+6-BA2.0mg/L+2,4- D0.1mg/L, for 3 weeks of dark treatment.8)The best medium composition for shoots induction from sterile seeding's stalk was Anderson +6-BA2.0mg/L, for a week of dark treatment.9) Activated charcoal was effective for preventing the explants from browning to a certain extent, 0.5g/L was the best initiation concentration.10) For transplanting of plantlets, the soil full of rotten leaves in the groves was the best material.the survival rate of plantlets being 85%.