The Isolation, Identification And Research Of Biological Property Of Lactobacillus From The Canine

Nowadays, the problems were obviously stood out such as the drug remaining, the drug resistant bacteria increasing ,etc, because of abusing antibiotic. The appearance of probiotics provided new idea for solving these problems.Two strains of bacteria were isolated from the rectal contents of 30 canines in this experiment. The two stains of bacteria were identified the species and genus which they belong to by their colony forms , thallus forms and biological properties , and the two strains of bacteria were Rod Lactobacillus , Swizerland Lactobacillus, respectively.The isolated Lactobacillus strains were passed the tests of tolerance to acid and bile salt, adherence to the epithelial cells of the canine intestinal mucous membrane, the inhibiting germ of filtering germs culture media and acute toxicity .It suggested that the isolated Lactobacillus strains can resist to the agents which were not fit for the growth of the germs in the animal intestinal environment, the above tests. And the isolated Lactobacillus could strongly adherence to the epithelial cells of canine intestinal mucous membrane. So the Lactobacillus can combine with the cells steadily so that the Lactobacillus could exist in the canine gastrointestinal tract, which prevent to be expelled from the body because of vermiculation of intestine .It suggested that the isolated Lactobacillus strains were no acute toxicity to the animal body from the acute toxicity test of the isolated Lactobacillus strains to 50 small Kunming white mice. And it suggested that the secretion of the isolated Lactobacillus strains could inhibit the familiar pathogenical germs from the inhibition germ test of the culture media of isolated Lactobacillus which removed the germs by filtered.The culture media of isolated Lactobacillus which were tested no toxicity to the animal body by acute toxicity test was fed to the young suckling canine .It can be found that the daily weight gain of the young controlled canine group was higher than that of the young compared canine group (it increased 14.76%), while the diarrhea rate of the youngcontrolled canine group was lower than that of the young compared group (it descended 6.22%).Above all, the isolated Lactobacillus strains are safe and no toxicity to the animal, can get to the canine gastrointestinal tract in losing very few , adhere to the epithelial cells of canine intestinal mucous membrane , which proved that the isolated Lactobacillus strains could steadily in the intestinal tract . The filtered removing germs culture media could inhibit the familiar pathogenical germs, so the isolated Lactobacillus could effectively inhibit the growth of harmful flora in the gastrointestinal tract. The obvious effects of weight gain and preventing diarrhea were obtained by the cultured media of isolated Lactobacillus feeding to the young suckling canine.In conclusion, the isolated Lactobacillus strains could be used as the micro-ecosystem preparation of probiotic for the canine.