Study On The Polymorphism Of GHR Gene In The Boer Goat

As we know, growth hormone receptor gene is one of the key genes which can influence the growth and development of animals. If there are polymorphisms on code region which will change amino acids of GHR gene, it'll alter the structure of growth hormone receptor gene, that will influence the combination between growth hormone and growth hormone receptor, and then it will alter the speed of animal growth and development. So, in order to detect polymorphisms on code region of growth hormone receptor gene , we studied the exon 4,5,6,7,10 of it in Boer goat with PCR-SSCP technique.In this study, firstly, on the basis of the sequences of GHR gene about goat on GeneBank, we designed the primers using the software named Primer 3 online (http://frodo.wi.mit.edu/) .Then, we amplified fragments with PCR technology and did agarose gel electrophoresis experiment to make sure the amplified fragments were the fragments what we wanted. Recovery them with fragment recovery kit and sequencing the mixture which mixed 8-10 samples. We can find whether there are polymorphisms in our target fragment and where they are according to the results of sequencing. If some polymorphisms were found ,then further study can be done such as genoryping with compatible enzyme and analyses their effects. There is no polymorphism found in the sequencing of the mixture. We did SSCP experiment in order to make sure it's bilievable. The two results of the study showed that there is no polymorphism in our samples.The main results were shown as follows:1. Results about exon 4: PCR fragment contains all sequences of exon 4, the primers was designed on the basis of AY739707 on GeneBank. The two sequences are completely the same between my sequence and AY739707.2. Results about exon 5: The primers were designed according to DQ179241 on GeneBank.The target DNA sequence was 204 bp long and contained the whole exon 5 sequences .The tatget sequences and exon 5 are the same.3. Results about exon 6: Prmers were designed according to DQ179242 on GeneBank. The whole exon 6 sequence was contained in the target fragment. The two sequences were all the same in length. 4. Results about exon 7: Prmers were designed according to DQ179243 on GeneBank. The whole exon 7 sequence was contained in the target fragment. The two sequences were all the same in length too.5. Results about exon 10: Prmers were designed according to DQ179245 on GeneBank. The target sequence is 549 basics long, and it is from basic 108 to 656 on exon 10.Fistly, we mixed several samples and sequenced it in order to detect polymorphisms. But we did not find any polymorphism. Then we did SSCP experiment to make sure there is no polymorphism in our samples indeed. The results of SSCP experiment showed that there is no polymorphism.