Flow Cytometric Studies Of Sorting For X-Bearing And Y-Bearing Sperm Of Boer Goat

The aim of these experiments was to study the sorting of X-bearing and Y-bearing sperm of boer goat. Fresh ejaculates were processed according to the following three treatments: Hoechst 33342 staining; Selection of percoll gradient centrifugation.; Flowcytometric sorting :1 ,The effects of Hoechst 33342 staining on boer sperm quality.spermatozoa (2×107) were incubated for 60 min with I 5.0mg/L, II 7.5mg/L, III10.0mg/L, IV12.5mg/L Hoechst 33342 dilution, viability >rate of viable and dead sperm , acrosome status and preservation of the spermatozoa were evaluated immediately after staining. Hoechst staining produces a significant reduction in spermatozoa quality when it is used in the dilution of III and IV.2, To study the effect of two-layer Percoll selection technique on normal morphology of boer goat spermatoza.the changes of sperm percentage of acrosoml integrity and percentage of swollen sperm before and after 45%-90% percoll gradient centrifugation were evaluated by Wright-staining and hypo-osmotic swelling test(HOST). Before and after the treatment,the changes of percentage of acrosomal intergrity (from 61.6% ± 14.5% to 84.6% ±12.4%; P<0.01) and percentage of swollen sperm(from 64.3%±8. 4% to 86.2%±7. 1%; P<0.01) were statistically significant. The spermatic functional parameters were distinctly improved after two-layer percoll gradient centrifugation. Consequently, Percoll gradient centrifugation can be applied in sorting program.3.Flow cytometric sorting for X-bearing and Y-bearing sperm Investigated the applicability of flow cytometric (FCM) sorting to select X-Bearing and Y-Bearing sperm of boer goat. The result of the separating rate is good and has reached 1000/s.