Effect Of Antioxidants On The Cells Of Macrobrachium Nipponense In Vitro

This paper presents results obtained on macrobrachium nipponense muscle cell and haemocytes in primary culture.To begin with, when vitamin C is 100mg/L, vitamin E is 200mg/L, Na2SeO3 is 150mg/L, NH4Cl is 150mg/L in M199 medium separately, muscle cell proliferation activity is the highest in subculture. The next, when muscle cell and haemocytes in primary culture are exposed to high ammonia-N, the production of superoxide anions (O2-) and the activities of antioxidant enzymes are caused to change by vitamin C, vitamin E, Na2SeO3 separately. The results showed as follow: (1) Vc group are higher than control group in the production of superoxide anions(O2-) and the activity of SOD of primary culture muscle cells ; Vc group is higher than control group in the production of superoxide anions(O2-) of primary culture haemocytes. but Vc group are lower than control group in the activities of SOD and GPX of primary culture haemocytes. Vc group is higher than control group in the activity of CAT. (2) Se group is lower than control group in the production of superoxide anions (02") of primary culture muscle cells. But Se group is higher than control group in the activity of SOD; Se group is lower than control group in the activity of SOD of primary culture haemocytes. But Se group is higher than control group in the activity of GPX of primary culture haemocytes; In addition, the activity of SOD is positive relation to the concentration of Na2SeO3 in primary culture muscle cells (3) VE group is lower thancontrol group in the production of superoxide anions (O2-) of primary culture muscle cells. But Se group is higher than control group in the activity of SOD; VE group are higher than control group in the production of superoxide anions (O2-) and the activity of SOD of primary culture haemocytes. (4) VESe group are higher than control group in the production of superoxide anions (O2-) and the activity of SOD of primary culture muscle cells; VESe group are higher than control group in the activities of SOD and GPX of primary culture haemocytes .