| Many researchers reported that the immune function of poultry, livestock and human could be damaged oweing to deficiency of selenium. While to supply animals with proper selenium could strengthen the response of cell immunity, humour immunity and non-specific immunity. But till now, few researches were seen about the effect of selenium on the immune function of rabbit. And few were done at one time with different selenium resources on the immunity of animals. The purpose of this study was to compare the effect of different selenium resources, sodium selenite, Kappa-selenocanageenan and DL-selenomethionine on the immune function and anti-oxidation of rabbit. In addition, the immune effects of sodium selenite, levamisole and Astragalus polysachain on the RHD freeze-dried vaccine were studied.1% Effects of different selenium resources on rabbit lymphocyte transformation stimulated by PHA in vitro. Different concentrations of selenium from 1 10-10MoL/L to lxlO"5MoL/L were added to the rabbit lymphocyte culture stimulated by PHA in vitro. Lymphocyte transformation was examined respectively at 24h, 48h with MTT colorimetric assay. Results indicated that at 24h, sodium selenite, Kappa-selenocanageenan and DL-selenomethionine could markedly stimulate the lymphocytes transformation with PHA at l 10-8MoL/L and 5 10-8MoL/L, l 10-7MoL/L , 1 10-6MoL/L and 5 10-6MoL/L (P<0.05) respectively. While at 24h, working on the lymphocyte from the same rabbit, DL-selenomethionine exhibited the best effect. It could promote the lymphocytes transformation at all the concentrations from l 10-10MoL/L and l 10-5MoL/L (P<0.05) , especially at l 10-5MoL/L (P<0.01). While Sodium selenite and Kappa-Selenocanageenan just at that of 1 10-7MoL/L and 1 10-6MoL/L ( P<0.05 ) , both exhibited inhibitory function at l 10-5MoL/L (P<0.05) .2, The reversion of different selenium resources on lymphocyte transformation restrained by inhibitor. We selected hydrocortisone and cyclophosphamide to build the model of immunosuppression in lymphocyte culture, while selenium added to the culture simultaneously with inhibitors or 3h before them. Lymphocyte transformation was examined at 24h with MTT colorimetric assay. The results revealed that pre-added selenium could effectively protect lymphocytes to resist and reverse the action of inhibitorsat most concentrations except l 10-5MoL/L of sodium selenite and Kappa-selenocanageenan. Among them, DL-selenomethionine showed the best, which could even promote lymphocyte transformation at proper concentration. When added simultaneously with hydrocortisone, DL-selenomethionine and sodium selenite at proper concentration could reverse the depressed lymphocyte transformation to the normal level, but Kappa-selenocanageenan had not the effect. When added simultaneously with cyclophosphamide, except 1 10-5MoL/L of Sodium selenite, Kappa-selenocanageenan, all other concentrations exhibited good effect, DL-selenomethionine was the best. 3% Effects of different selenium resources on the function of rabbit humour immunity and anti-oxidation in vivo. Thirty-five rabbits were randomly divided into seven groups and vaccinated with RHD freeze-dried vaccine. At same time, the rabbits were injected respectively with sodium selenite (0.1 mg/kg and 0.3 mg/kg) , Kappa-selenocanageenan (0.1 mg/kg and 0.3 mg/kg) , DL-selenomethionine (0.1 mg/kg and 0.3 mg/kg) and physiological salt solution as control. Antibody against RHD, activity of GSH-Px and content of MDA in rabbit serum were detected on 0, 10, 20, 30d after inoculation. Results showed that sodium selenite (0.1 mg/kg) , Kappa-selenocanageenan (0.3 mg/kg) , DL-selenomethionine (0.3 mg/kg) could increase the level of RHD antibody, while sodium selenite (0.1 mg/kg and 0.3 mg/kg) , Kappa-selenocanageenan (0.1 mg/kg and 0.3 mg/kg) improved the activity of GSH-Px. Kappa-selenocanageenan (0.3 mg/kg) , whereas, represented good effect on eliminating the MDA in serum.4, Effects of different adjuvants of RHD freeze-dried vaccine on the immune function of rabbits. Twenty rabbits were r... |
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