Screening Of Chinese Herb Medicines And Pharmacodynamic Studies On Their Active Components For The Treatment And Prevention Of Bovine Mastitis

Chinese herbs of Flos lonicerae, Herba taraxci, Fructus forsythiae, Radix trichosanthis, et al wereselected by cylinder plate assay of microbiological method for preventing and curing clinical mastitis ofdairy cows and some of their pharmcodynamic characteristics were studied in this paper. The results showed that the improved method of Sorbitic was the best method to extract chlorogenicacid from Flos lonicerae. Chlorogenic acid in extracted material was analyzed qualitatively andquantitively by UV, TLC and HPLC.Result of UV showed that it imply 1.26% chlorogenic acid in Floslonicerae. The extracted material of Flos lonicerae was analyzed by TLC and it implied 90% chlorogenicacid. Herba taraxci amylose was extracted by various solvents (petroleum ether, aether, ethanol, water, etal.) and analyzed qualitatively and quantitively by the colorimetry of hydroxybenzene-vitriol. It implied1.0056% herba taraxci amylose in Herba taraxci. The extracted material of Herba taraxci was analyzed byUV and it implied 86% herba taraxci amylose. Forsythiaside was extracted by percent 75 ethanol fromFructus forsythiae with the improved method of sorbitic and separated by VLC and methanol-chloroformcommixture of different concentration and detected by TLC. Rf was 0.35 place which had a strongerspot of fluorescence. It showed that there were forsythiaside. Five medicines include chlorogenic acid(CHA), herba taraxci amylose(HTA), forsythiaside(F),mixture of chlorogenic acid, herba taraxci amylose and forsythiaside(CFH), and ethanol extraction ofcompound of Flos lonicerae, Fructus forsythiae and Radix trichosanthis(JLT) for pharmacodynamic tests.Results of antibacterium tests showed that F had stronger action against pathogenic bacteria . Of F andCHA, MIC and MBC to Staphylococcus aureus were lower than those of others. CFH had no antibacteriumaction on Streptococcus dysgalactiae, while HTA had antibacterium action only on Strptococcusdysgalactiae with certain degree of MIC but no action on other two pathogenic bacteria. Results ofanti-inflammation test showed that Under 10mg/kg, only JLT and CFH had anti-inflammation action to theinflammation model of xylene in five medicines. Results of the particularity immunity test showedthat all medicines could increase notably antibody level of chicken red blood cells except for HTA.Results of the non-particularity immunity tests showed that all medicines had the weaker action tothe viscera index of immunity organ. There was not significant difference to compare with N.S while therewas significant difference to compare with Cy (P<0.05). F could increase the ability of macrophageengulfing chicken red blood cells. HAT, F would enhance the multiplication of lymphocyte.