| In order to study on antibacterial medicine against mastitis of dairy cattle, four kinds of Chinese herbs with antibacterial function were chosen according to "Veterinary Pharmacopoeia of PRC" and a great deal of interrelated references on Chinese herb medicines. They were Chrysanthemumin-dicum L, Andrographis paniculata (Burm. f.) Nees, Isatisindigotica Fort and Fructus chebulae. Their extracting processes were optimized by orthogonal test and six herb extracts were gotten. Six herb compounds were made on the base of some classic prescriptions. Three common western veterinary medicines were fixed with the former twelve extracts and compounds. Then their antibactatic effects were determined through antibiotic test in vitro.The results showed that most of the herb extracts and compounds can inhibit the growth of Bacillus subtilis and Escherichia coli, but their effects against Staphylococcus aureus were different. Among them, the ethanol extracts of Andrographis paniculata (Burm. f.) Nees and Fructus chebulae extracts had certain antibiotic activity against three bacteria.The bacteriostatic activities of these herb compounds were different. Bacillus subtilis and Staphylococcus aureus were sensitive to most of them, and Escherichia coli only to 11~#. Staphylococcus aureus is the main pathogen causing mastitis of dairy cattle, so 10~#, 11~#, and 13~# are chosen as the objects of further study.The bacteriostatic activity of the herb compounds were strengthened after combinated with three western veterinary medicines, however, the different combination of western veterinary medicines had different inhibition to the three bacteria. 7~#, 8~#, 9~# and 11~# with Ampicillin Sodium, 1~#, 2~#, 3~# and 10~# with Sulfadiazine Sodium, 2~#, 4~#, 7~# and 11~# with Ciprofloxacin Hydrochloride Monohydrate were chosen for further study.
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