| In vitro, the adsorption ratio of AAN on aflatoxins B1, B2, G1, G2 in phosphate buffer saline at pH 7.4 and 37C are 89.81%, 82.23%, 85.24%, 78.42% respectively ; and the adsorption ratio of MS on aflatoxins B1, B2, G1, G2 on the same condition are42.36%, 39.51 %, 41.75%, 34.56%, respectively.The other trial was conducted to evaluate the effect on AAN and MS on adsorbing aflatoxins B1, B2, G1, G2 in vivo. Four hundred and eighty Avian broiler chicken (seven days old) were randomly assigned to the following treatment groups: (1) basal diet only (control); (2) 0.3%(w/w) MS; (3) 0.3%(w/w) AAN; (4)122.7ugAF/kg of diet; (5) 0.3% (w/w) MS+122.7ugAF/kg of diet (6) 0.3%(w/w) AAN+122.7ugAF/kg of diet. Each treatment consisted of three pens of twenty chicken/pen. Feed and water were provided for adlibitum consumption. At seventy days of age, the study was terminated and twelve chicken (three replicates of four each) from each treatment group were bled by carotid artery for serum biochemical analysis.After bleeding, chicken were killed by cervical dislocation to determine carcass characteristics, all samples needed were collected.The AFBj residue in muscle in group 4, 5, 6 were 2.74+0.39ng/g, 1,72+0.36ng/g and 1.51+0.31ng/g, respectively. The AFB1 residue in muscle in group 6 and group 5 were decresed by 44.89% (P<0.01) and 37.23% (P<0.01), as compared to group 4, respectively.The AFB1 residue in liver in group 4, 5, 6 were 7.23+0.88ng/g,2.06+0.25ng/g and 0.97+0.14ng/g, respectively. The AFB1 residue in liver in group 6 and group 5 were decresed by 86.58%(P<0.01) and 71.51%(P<0.01), as compared to group 4,respectively.The feed trial showed that the addition of AF to the diet decreased ADG by 9.56% (P<0. 05), as compared with control (AF6.5 ng/g) .The biochemical analysis showed that the ALB levels in serum in group5 and group6 were increased by 19.83%(P<0.05) and 20.45%(P<0.05), as compared with control(AF127ng/g), respectively; the IgA levels in serum in group5 and group6 were increased by 20.81% (P<0.05) and 23.12% (P<0.05). as compared with control (AF127ng/g), respectively; the IgG levels in serum in group5 and group6 were increased by 22.22% (P<0.05) and 24.87% (P<0.05) . as compared with control (AF127ng/g) , respectively; the IgM levels in serum in group5 and group6 were increased by 47.91% (P<0.01) and 58.33% (P<0.01), as compared with control (AF127ng/g) , respectively. The GR levels in serum in group 5 and group 6 were decreased by 13.99% (P<0.05) and 15.27% (P<0.05), as compared with control (AF127ng/g), respectively; the GST levels in serum in group 5 and group 6 were decreaied by 19.62% (PO.05) and 20.24% (PO.05), as compared with control (AF127ng/g), respectively; the ADA levels in serum in group 5 and group 6 were decreased by 23.23% and 23.91%, as compared with control (AF127ng/g), respectively; the GR levels in liver in group 5 and group 6 were increased by 29.95% (P<0.05) and 32.62% (P<0.05), as compared with control (AF127ng/g), respectively; the GST levels in liver in group 5 and group 6 were increased by 20.76% (P<0.05) and 21.47% (P<0.05), as compared with control (AF127ng/g), respectively; the ADA levels in liver in group 5 and group 6 were increased by 34.66% and 36.36%, as compared with control (AF127ng/g), respectively. The GPT levels in group 5 and group 6 were decrease by 34.61% (P<0.01) and 35.61% (P<0.01), as compared with control (AF127ng/g), respectively; the GOT levels in group 5 and group 6 were decrease by 21.94%(P<0.05) and 20.98%(P<0.05), as compared with control (AF127ng/g), respectively; the AKP levels in group 5 and group 6 were decrease by 23.87%(P<0.05) and 24.18%(P<0.05), as compared with control (AF127ng/g), respectively. |
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