| The purpose of experimental program was to research the effect of Xylo-oligosaccharides and Pribiotics on performance, intestinal bacterium and serum indexes of different stage calves, according to the differet level of Xylo-oligosaccharides and Pribiotics fed on calves in different stage. The experiment give a example about applance of Xylo-oligosaccharides and Pribiotics on performance of rumen animals.Trial 1 The effects of Xylo-oligosaccharides on performance and serum indexes in dairy calves. A total of 15 dairy calves with 7-day-old were divided into 3 groups with 5 in each group. Each group was accorded to the principle of body weight, birth similarity and weaned at 60days. The first group was control group, the second and the third group fed on the same diets which were added 5g/d and 10g/d XOS one calf. There were no significantly difference among groups of newborn calves weight (p>0.05). Each group was fed the same milk, calf starter, hay, and nutrient composition in 42 days. The experiment was conducted to investigate the effect of supplementation with XOS on performance, the bacterium populations, immunization and metabolism of lipid in calves. The result indicated that the ADG of 5g/d group was significantly higher than control group and 10g/d group (p<0.05), and the 10g/d group has not significant difference (p>0.05) compared with control group. The index of diarrhea control group, 5g/d group and 10g/d group were 3.8%, 0.4% and 1.9% respectively. The index of diarrhea 5g/d group and 10g/d group were a little lower than that in control group, and the index of diarrhea 5g/d group was a little lower than that in 10g/d group. The other result showed that the decrease in E.coli population (8.61,8.93,8.92) and increase in lactobacillus population (8.47,8.42,8.15) in 5g/d and 10g/d groups were better than those in control group and has not significant difference (p>0.05) among all groups. The CHOL of 5g/d group was significantly higher than control and 10g/d group (p<0.05), the HDL, LDL, TG, ALB, GLOB, TP, IgA, IgG, IgM, BUN and GLU have not significant difference (p>0.05) among all groups. Considering all factors, in this experimental condition, 5g/d and 10g/d groups fed on calves were better than control group, and 5g/d group was better than 10g/d group.Trial 2 The effects of Probiotics on performance and serum indexes in dairy calves. A total of 16 dairy calves with 70-day-old were divided into 2 groups with 4 in each group. Each group was accorded to the principle of body weight, birth similarity and weaned at 60days. The first group was control group, the other group fed on the same diets which were added 0.2% Probiotics (live bacterial population higher than 9.6×10~9CFU/g). Each group was fed the same milk, calf starter, hay, and nutrient composition in 35 days. The experiment was conducted to investigate the effect of supplementation with Probiotics on performance, the bacterium populations, immunization and metabolism of lipid in calves.The result indicated that the bodyweight of trial group was significantly higher than control group (p<0.05) in Holstein calves, and the bodyweight of trial group was not significant difference (p>0.05) compared with the control groups in Jersey. The ADG of trial group in Holstein and Jersey calves were higher than that of control group(p<0.05). The other result showed that the decrease in E.coli population and increase in lactobacillus population in trial group was better than those in control group and has not significant difference (p>0.05) in two groups. The CHOL, HDL, LDL, TG, ALB, GLOB, TP, IgA, IgG, IgM, BUN and GLU have not significant difference (p>0.05) among all groups. Considering all factors in this condition, trial group fed on calves were better than control group, which improved the performance and maintained the microecosystem balance of calves.
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