Survey Of Ectomycorrhizal Fungus Flora Association With Picea Mongolica And Study On The Mycelial Culture Condition Of Cenococcum Geophilum Fr.in Baiyinaobao

Picea mongolica is a rare and endangered species in the world, which is conferred to the tide of "biology gene bank" and "living fossil" , and mainly distributes in Baiyinaobao sandy land of Chifeng. Picea mongolica seedlings have difficulty in forming mycorrhizae in nursery, Maybe because there are not corresponding effective ectomycorrhizal fungi. Therefore, a preliminary survey has been performed for ectomycorrhizal resource of Picea mongolica and Betula Platyphylla in Baiyinaobao Region. The survey shows that there are altogether 7 different ectomycorrhizal fungi which are subordinated to 5 families and 7 genera. These ectomycorrhizal fungi are Cenococcum geophilum (C.g), Boletus reticulatus, Leccinum scabrum, Russula aeruginea, Lactarius akahatus, Ramaria flava, Amanita muscaria.By isolating those ectomycorrhizal fungi from fruitbody, mycorrhizae rootlet and scleorotia, we got 9 strains. In the course of investigation, we find that C.g is a predominant ectomycorrhizal fungus in this region. The culture condition of 2 C.g strains which had been isolated from mycorrhizal tissue and scleorotia under Picea mongolica has been tested. These two strains was respectively nominated C.g-ysg and C.g-ysh.The experimental results show that: the best culture media for C.g-ysg is MRD, and the another is Pachlewski; Glucose and KNO₃ arc-respectively the best C and N source for C.g-ysg, but the best C and N source for C.g-ysh are respectively sucrose and KNO₃; C.g-ysg can grow normally at the range of experimental pH value 4⁹, and C.g-ysh can also grow normally between experimental pH value 4⁷. All these results will provide the theory basis for the reproducing of C.g inoculum.