Ecological Distribution Of Cenococcum Geophilum In Typical Forest In Inner Mongolia

The study is focused on Cenococcum geophilum Fr. (Cg for short) of the typical mountainous forest vegetation in Inner Mongolia. The objective of this study is to find out the influence and the distribution of the mycorrhizal fungi. A comprehensive and systematical investigation and research was conducted in the following five study areas: Daxing'anling natural Larix gmelini forests, Black River the mixed needleleaf and broadleaf forest, natural secondary forests of Betula Platyphylla and Populus davidiana of Daqing Mountain and Ural Mountain, Helan Mountain Picea crassifolia Nature forests. The main results are as follows:l.The study on Cenococcum geophilum in these five study areas shows that the fungi species we studied is able to establish symbiotic relationships with all of the dominant species of these regions. The study also shows that those from different study areas share similar morphological characteristics, most of which are black rod shaped, but the amount of exterior mycorhiza hyphae, the present set of the fungi, and the thickness of mycorhiza are uneven, between the broadleaf and needleleaf tree species, and between shrubs and arbors.2. The study shows natural infection rates of Cenococcum geophilum and rhizosphere soil properties correlation analysis shows that natural infection rates of Cenococcum geophilum and a rhizosphere soil with available N and K were positively correlated, and soil pH was negatively associated, but did not reach significant level (P> 0.05), soil organic matter, total nitrogen was positively correlated (P<0.05). available P was significantly correlated (P<0.01). Natural infection rates of Pinus tabulaeformis forests in Daqing Mountain, Ural Mountain nature reserve and Juniperus rigida forests Helan Mountain nature reserve are below average, while the rest study area are over 30%.3. The study on the distribution and seasonal dynamics of Cenococcum geophilum living in native artifical pine plantation in Daqing Mountain has shown that Cenococcum geophilum is mainly distributed within the middle and lower plots of the shady slopes,but none of this fungi species was found on sunny slopes and the upper plot of the shady slopes. 4. The selected strains, from the morphology point of view, were tapetum-like or villous, with the colors of brown or dark brown. Growth rate of mycelium of different strains are also different. According to colony color, morphology initially identified as Cenococcum geophilum. To 11 strains of mycelia material, DNA extraction, were determined to identify strains of the species they identified. Measured in this study, samples of the 11 strains and GenBank sequences in Cenococcum geophilum base sequence similarity of 96% to 99%, and high homology, so the space group identified as Cenococcum geophilum.5. Synthesis and drought resistance of pine seedlings test results showed that the growth of seedlings are significantly promote. Under drought stress, conifer seedlings with the critical drought occurs and wilting come out late, chlorophyll content and proline content were increased by 43% and 47.6% respectively. It is indicted that Cenococcum geophilum can improve the drought resistance of pine seedlings.