Study On The Liquid Culture Condition Of Ectomycorrhizal Fungus-Cenococcum Geophilum And Its Nutrient Physiology

Cenococcum geophilum Fr.(Cg) is one of the superior micorrhizal fungi in Mount Daqingshan, Inner Mongolia Autonomous Region. This paper studied the nutrient physiological characteristics and the liquid culture condition of Cg rather completely. By studying the growth speed on its culture media and the growth pattern, we selected the most appropriate one as the culture media for Cg and detailed in the selection experiments of pH value, carbon and nitrogen source, the growth period, inoculating amount and the capacity of oxygen accommodation. The experimental results show that the best culture media for Cg is pachlewski. At its liquid fermentation culture process, the most appropriate carbon source is glucose and white sugar, while the most appropriate nitrogen source is Ammonium Tartrate .The results also indicate that Cg grows best between pH value 6.0-8.0,in which its growth period is about 15 days, and the best inoculating amount and the liquid capacity are respectively 10-15% and 100-200ml.All these results will provide the theory bases for the reproducing of cenococcum geophilum.