Study On Rapid Multiplication Procedure Of Almond (Amygdalus Communis L.)

From the study, we have developed the rapid multiplication procedures of three kinds of Almond(Amygdalus　communis　L.)-Nongmei, Chaomei and Meixin had been researched, including the suitable cultivation about the sterilization of explants, establishment of aseptic cultures, selection of suitable medium and hormone in proliferation and strengthen of shoots, avoidance of etiolated plantlet, rooting, domestication and transplanting. All work and conclusions or results are below: 1. Preceding treatment of explants. The survival rate and the pollution rate of explants were statistic index. It revealed that soaking explants by alcohol (75%, m/m) for 30 seconds and HgCl₂ (0.2%, m/m) for 11 minutes, 9 minutes and 11minutes separately can sterilize explants well. 2. Establishment of aseptic cultures. The inducing rate of explants was statistic index. It revealed that G medium was suitable for Nongmei, MS medium was suitable for Chaomei and Meixin. 3. Proliferation of shoots. Proliferation and effective branch rate of explants were statistic index. It revealed that G+6-BA 1.0mgï¹’L^-1 was suitable for the multiplication medium of Nongmei, G+6-BA 1.0 mgï¹’L^-1 and G+6-BA 0.5 mgï¹’L^-1+IBA 0.125 mgï¹’L^-1 were suitable for the multiplication medium of Chaomei, MS+6-BA 1.0 mgï¹’L^-1 was suitable for the multiplication medium of Meixin. 4. Strengthen of shoots. Effective branch rate and growth state of explants were statistic index. It revealed that G+6-BA 0.25 mgï¹’L^-1+GA30.5 mgï¹’L^-1 was suitable for Nongmei,G+6-BA 0.5 mgï¹’L^-1+IBA0.125 mgï¹’L^-1 was suitable for Chaomei, MS+6-BA 0.25 mgï¹’L^-1+GA30.5 mgï¹’L^-1 was suitable for Meixin. 5. Avoidance of etiolated plantlet. The exchange culture of MS medium and G medium had better effect than only one medium for the delay of the appearing time of etiolated plantlet, and it could acceleration the proliferation and growth of shoots. 6. Rooting. Rooting rate and rooting number of explants were statistic index. It revealed that B5+ IBA140 mgï¹’L-1 +Citric Acid 200 mgï¹’L-1+AC 0.1%, B5+ IBA120 mgï¹’L-1 + Citric Acid 200 mgï¹’L-1+AC 0.1% and B5+ IBA130 mgï¹’L-1 + Citric Acid 200 mgï¹’L-1+AC 0.1% were suitable inducing root media for Nongmei, Chaomei and Meixin respectively; B5 +AC 0.1% was suitable rooting medium. 7. Domestication and transplanting. The key way was that the domesticated plants should be transferred in sterile from flasks to pots as matrix of peat, with irrigation of nutritive solution and with moisture control.