The Elementary Study On Molecular Genetic Markers Of Sheep Meat And Wool Traits

Molecular genetic marker can be applied to animal breeding with the development of the molecular biology technique. The elementary study on molecular genetic markers of meat and wool traits of sheep existing in Xinjiang was carried out by the technique of molecular genetic marker. The buttocks muscle can be increased about 30% by Callipyge (CLPG), resulting from an apparent A-->G mutation in 32.8 kb upstream of GTL2 in the ovine chromosome 18. The mutation polymorphism is referred to as SNPCLPG, which was later shown to be the only difference between the callipyge and wild-type chromosome. The objective of the first research is to select carriers of the callipyge gene by the molecular biological techniques, which is the basis of selection of the excellent meat traits. The SNPs were detected by PCR-restriction fragment length polymorphisms (PCR-RFLP) and PCR-single-strand conformational polymorphism (PCR-SSCP) with two pairs of primers in genome of Australian Dorset sheep, Germanic Merino sheep and Dorset×Chinese Merino inbred F1-2 muscle sheep. No A—>G substitution was found though two special products (165pb and 497bp) were amplified. In the second research, 29 pairs of microsatellite primers were synthesized for the microsatellite markers linked to fineness and the genome were detected by Touch Down PCR and PAGE in Xinji fine wool sheep, Chinese Merino sheep( Xinjiang) and Kazakh sheep. 9 microsatellite markers with polymorphism were selected after preparatory research. 3 microsatellite markers including MAF64,OarDB6 and KAP8 were used to analyze the genome Xinji fine wool sheep, and Allele frequency (N), Polymorphism information content (PIC) and Heterozygosity (H) were calculated. The results demonstrates that three the microsatellite loci are high polymorphic in this flock and they may be related to wool finesse.