Study Of Pathogen Isolation And Rapid Detection By Polymerase Chain Reaction (PCR) On Cow Brucellosis

In order to ascertain the infected situations and epidemic characters of brucellosis of cows in Inner Mongolia, we did the serologic detection of the cow that had the symptom of Brucellosis, such as abortion, premature delivery, fetal death, placental retention. All those cows were raised in different scale dairy. Meanwhile, to ascertain the strains and biotypes of Brucella, the aborted tissues and milk of positive serological cows were collected. In the study, we examined as much as 642 cows that were bred in different dairy and districts by method of serologic detection. The results indicated that the average positive rate of RBPT(Rose Bengal Plane Test) was 39.39%, SAT (Standard Tube Agglutination Test)was 31.05% and Eenzyme Linked Immunosorbent Aassay (ELISA) was 60.84%. The results revealed that not only there were Brucellosis in Inner Mongolian, but also the epidemic situation increased again. It was popular in some districts and even it pervaded seriously in a few ones. 101 positive cows were detected in total and 2 strains of type 3 Brucella were isolated.For making up the some deficiences of serologic diagnosis and microbiological examination, the author also set up a method to dectect Brucellosis by Polymerase Chain Reaction(PCR). The result showed that the expected gene with 384bp was amplified based on the primer which designed according to the sequence of gene encoding the outer membrane protein (31KDa). Besides its simpliness and quickness, the method also has characters of specificity and sensitivity. It has a great importance to detect and diagnose Brucellosis by Polymerase Chain Reaction (PCR).