Cloning And Analysis Of Calmodulin Gene From Salvia Miltiorrhiza Bunge And Vigna Unguiculata (L.)Walp

Calmodulin is a kind of small molecular acid protein with heat resistance, pI:3.9-4.3. Calmodulin is highly conserved in eukaryotes and a multifunctional protein which has an important function in Ca²⁺ signal system. Calmodulin takes part in many aspects: enzyme activity regulation,kinases response,cell division and differentiation,cell lines and movement,cell secretion and infiltration, photosynthesis, photomo rphogenesis,ion absorption and transit,plant reproductive and fertility,hormone response,cell membrane channel and Gene expression regulation etc. By now, lots of calmodulin genes have been cloned from many species: barely,soybean,wild cabbage,arabidopsis thaliana,maize.Salvia miltiorrhiza Bunge,a perennial plant, is a traditional herb in China. It has lots of medicine effects: curing heart and cerebrovascular diseases,anti-tumor,resisting to inflammation,anti-microbiologicaldiease,protecting from oxidation, slowing oldness,renewing etc. Traditional herbs, belonging to an important part of Chinese knowledge, are used for thousands of years in China. Vigna unguiculata (L.)Walp is a very useful vegetable for humans. Vigna unguiculata (L.)Walp contains lots of nutritions, such as proteins,sugars,phosphorous,iron,calcium,vitamins and food fibers. Calmodulin from Salvia miltiorrhiza Bunge and Vigna unguiculata (L.)Walp have been not reported.Total RNA was isolated from leaves of Salvia miltiorrhiza Bunge and Vigna unguiculata (L.)Walp. There are four methods used in RNA isolation from Salvia miltiorrhiza Bunge: the method of Guanidine thiocyanate> the method of CTAB , the method of SDS , the method of total Nucleotide acid isolation. Compared with other methods, the method of Guanidine thiocyanate was considered as a method with simple steps and the good result. By analyzing different factors in isolation, it is concluded that: the...