The Variations Of Anthocyanins Among Some Speceis And Cultivars Of Section Camellia And Their Application On Classification

At present, there are many disputes in the taxonomy systems of Genus Camellia L. in the world, of which Sect. Camellia is one of the arguments This is because these systems mainly depend on the phenotypes of these plants. The chemotaxonomy with red pigments (anthocyanidins and anthocyanins) of flower petals of Sect. Camellia as markers not only brings new evidences to phylogenensis and classification of sect. Camellia plants, but also plays an important role in classification of cultivars and assistant breeding method for color objectives.The representative sampling florescence of single flower should be collected in the suuny directions and outside canopy above the middle position during the blooming period through semi-opening to full opening for pigments analysis. The petals both treated with boilling water and then dried by silica,or directly dried by silica are good nought to be used for pigment analysis when the materials collected in a long distance from the laboratory, while the fresh petals are the best to be extracted when near the laboratory.TLC can be used as an assistant method in pigments component analysis by silica gel G plate and Xin Hua No. 3 filter paper. The HPLC methods were well determined for two kinds of pigments including red petal pigments of species and cultivars of Sect. Camellia and yellow petal pigments of a cultivar which does not origin from C nitidissima. The sequence of the 13 known pigments out of HPLC as peaks is (Cy3G5G< Pg3G5G)< (Cy3Ga< Cy3GG