Study On Reproduction Biology And Genetic Diversity Of Cardiocrinum Giganteum

Cardiocrinum giganteum has not been exploited. This research has studied two aspects of Cardiocrinum giganteum:(1) the reproduction biology of Cardiocrinum giganteum, including sexual reproduction and bulbs reproduction;(2) The genetic diversity of 5 natural populations of Cardiocrinum giganteum were analyzed by using random amplified Polymorphic DNA (RAPD) markers. The genetics condition of populations were investigated from the DNA level. The results were as follows:1. The effects of light and temperature on seed germination of Cardiocrinum giganteum was studied in this experiment. The results showed that light obviously improved the seed germination of Cardiocrinum giganteum, it enhanced the percentage of its germination. The optimum condition to seed germination were 24h illumination and 20℃.The result also revealed that the seed germination was influenced by temperature to Cardiocrinum giganteum in the dark treatment. The optimum temperature to seed germination was20℃.Germination began 11 days and ceased 3 weeks after the initiation of the experiment. The percentgae of seeds germination was decreased with the change of temperature. Seed germination was also influenced by the seed location in the fruit, in which seed in the middle was superior to the lowst and the upset. Seed germination had positive correlation to the weight of a thousand seeds.2. Through observation and analysis of the cultivation of bulbs from different origins. The results indicate that Cardiocrinum giganteum bears the low temperature ability to be strong, All plants can safely survive the winter in the field. Bulb's size was the key of the flower numbers and bulb regeneration numbers.3. The genetic diversity of 5 natural populations of Cardiocrinum giganteum were analyzed by using RAPD markers. Totally 104 bands were detected with 14 primers, Eighty-seven polymorphic bands were detected, representing of 86.54% of polymorphic bands(PPB).Among 90 samples ,the RAPD-based genetic similarity ranged from 0.2727 to 0.9091,with the mean of 0.5698;the genetic distance ranged from 0.2082 to 2.6224,with the average of 0.6948, showed Cardiocrinum giganteum has great rich genetic diversity. Cluster analysis by unweighted pair group method of arithmetic means (UPGMA) suggested that 90 samples could be divided into five groups with genetic similarity from 0.5698. One sample from Baishuihe was the most different, so it was shown a separate group.4. It shows that genetic similarity coefficient between Hongkou, Lianghe, Baishuihe, Wangyu and Kangding is 0.5808,0.5915,0.6156,0.6625,0.6153; genetic distance is 0.6493,0.6455,0.6200,0.4695,0.5558. Each population could be divided into two groups, only the genetic diversity of Hongkou populations based on RAPD markers was correlated with their geographical distribution. UPGMA cluster analysis showed that the distribution of Cardiocrinum giganteum has two kinds of situations, mottling distribution and stochastic combination. This research indicated that it was very effective to set up the nature protection area for protecting the genetic diversity of Cardiocrinum giganteum.