Effect Of Gehua Decoction On Immune Inflammatory Factors And Expression Of APC In Transplanted Hepatocellular Carcinoma Mice

Object:This topic is in the Gehuajiecheng decoction as the research object, on the base of the previous research, through the establishment of transplanted liver cancer in mouse model, observe the Gehuajiecheng decoction antitumor effects, the effects on the immune inflammatory factor expression and colorectal adenomatous polyp protein(APC) tumor cells。Methods:The male BALB/c mice with experiments were inoculated with H22tumor-bearing, gastric lavage of Gehuajiecheng decoction, after twelve days treatment, removal of the eyeball blood, after centrifugation, the supernatant; After the mice were sacrificed, take Spleen、Thymus、tumor mass, tumor block fixed with4%paraformaldehyde。Spleen and thymus weighed, calculation the Spleen index and thymus index; Tumor mass weighed, calculation the inhibition rate; Enzyme linked immunosorbent assay was employed to determine the amount of interleukin1(IL-1)and necrosis factor α (TNF-α) in serum。The protein expression of APC were evaluated by SABC immunohistochemistry。Results:(1)The effect of Gehuajiecheng decoction to IL-1inperip-heral blood:Model、high and low dose of Gehuajiecheng decoction groups compared with blank group have significant difference(p<0.05);(2)The effect of Gehuajiecheng decoction to TNF-α inperipheral blood:Model、 high and low dose of Gehuajiecheng decoction groups compared with blank group have significant difference(p<0.05);(3)The effect of Gehuajiecheng decoction to APC inperipheral blood:High and low dose of Gehuajiecheng decoction groups compared with model group have significant difference(p<0.05)。Conclusion:Gehuajiecheng decoction can reduce the level of IL-1and TNF-a in certain dose, on the basis can reduce the APC positive expression rate。The anti-tumor mechanism of Gehuajiecheng decoction maybe concerned with adjust the level of IL-1and TNF-α、improve the function of immune system and inhibiting the gene expression of APC。...