Study On The Correlation Between Cytotoxic T Cells And Inhibitory T Cells And Hemodialysis Syndrome Of Psoriasis Vulgaris

Background Psoriasis is a common,chronic,inflammatory skin disorder. Chinese medicine means that the main pathogenesis of psoriasis is "Blood heat", which is one of its basic syndromes,and its essence remains unknown and need to further study.Abnormal activation of T cells play an important role in the pathogenesis of psoriasis.While Tc and Ts cells is closely related to the activation of T cells, but the the relationship between psoriasis and both Tc andTs is unclear. According to its activation stage, T cells are divided into effector T cells, central memory T cells, effect memory T cells. But the levels of the activation and proliferation of Tc and Ts cells in the peripheral blood of patients suffering from psoriasis with blood heat have not been reported. Therefore,in order to reveal the immunopathogenesis of psoriasis with blood-heat syndrome and its modern medicine material basis of blood-heat syndrome.lt has important theoretical and practical significance for discovering the pathogenesis of psoriasis to investigate the activation and proliferation level of Tc andTs cells in peripheral blood of patients with psoriasis of blood-heat syndrome in vivo.Objective In order to explor the activation and proliferation levels of Tc andTs cells and those cells in different activation stages,and in order to study the possible role of Tc andTs cells in the pathogenesis of psoriasis with heat type. We detected the expressions of activation factor CD38 and HLA-DR,and proliferation related factors Ki-67 in Tc cell and Ts cells of peripheral blood of psoriasis with heat type.Methods The expression proliferation related factor Ki-67 and activation related factor CD38 and HLA-DR of Tc cell and Ts cells in peripheral blood of psoriasis with heat type of psoriasis and healthy group was investigated by polychromatic flow cytometry.Results1.The results about Tc cell1.1 Tc cellThe percentage of Tc cell (67.98±17.17)% in psoriatic patients with blood-heat syndrome, and was significantly up-regulated relative to (51.05±19.37)% of the control group, a statistical difference was found in both aspects (P＜0.05); The percentage of CLA+Tc cell (7.71±10.77)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (10.84±16.84)% of the control group;The percentage of Tc cell express activation-related factors CD38+HLA-DR+was (16.67±10.45)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (14.87±9.83)% of the control group;the percentage of expressing Ki-67+was (60.52± 22.35)%, and no significant difference (P＞0.05) compared with (7.55±19.27)% of the control group.1.2Effector Tc cellsThe percentage of effector Tc cells (9.02±8.16)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (10.77±8.17)% of the control group. The percentage of effector Tc cell express activation-related factors CD38+HLA-DR+was (22.78±27.18)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (15.78±19.18)% of the control group;the percentage of expressing Ki-67+was (19.14±25.85)%, and was significantly up-regulated relative to (11.00±19.05)% of the control group, a statistical difference was found in both aspects (P＜0.05); The percentage of CLA+effector Tc cell express activation-related factors CD38+HLA-DR+was (22.57±14.61)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (16.00±7.53)% of the control group;the percentage of expressing Ki-67+was (31.71±22.29)%, and no significant difference (P＞0.05) compared with (20.40± 14.75)% of the control group.1.3 Central memory Tc cellsThe percentage of central memory Tc cells (18.80±12.11)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (20.80±14.99)% of the control group. The percentage of central memory Tc cells express activation-related factors CD38+HLA-DR+was (19.11±15.98)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (17.08±13.68)% of the control group;the percentage of expressing Ki-67+was (15.47±29.53)%, and no significant difference (P＞0.05) compared with (8.71±21.21)% of the control group. The percentage of CLA+ central memory Tc cells express activation-related factors CD38+HLA-DR+was (67.44±36.89)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (53.39±43.64)% of the control group;the percentage of expressing Ki-67+was (59.07±29.54)%, and no significant difference (P＞0.05) compared with (42.30±31.30)% of the control group.1.4 Effect memory Tc cellsThe percentage of effect memory Tc cells (25.11±24.04)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (21.83±19.71)% of the control group. The percentage of central memory Tc cells express activation-related factors CD38+HLA-DR+was (19.80±15.27)% in psoriatic patients with blood-heat syndrome, and was significantly up-regulated relative to (11.81±17.03)% of the control group, a statistical difference was found in both aspects (P<0.05);the percentage of expressing Ki-67+was (11.15±11.30)%, and no significant difference (P＞0.05) compared with (8.05±8.26)% of the control group. The percentage of CLA+central memory Tc cells express activation-related factors CD38+HLA-DR+was (21.11±15.77)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (18.64±17.39)% of the control group;the percentage of expressing Ki-67+was (9.48±14.56)%, and no significant difference (P＞0.05) compared with (9.39±8.95)% of the control group.2. The results about Ts cell2.1 Ts cellThe percentage of Ts cell (33.45±21.21)% in psoriatic patients with blood-heat syndrome, and was significantly decreased relative to (47.94±2.39)% of the control group, a statistical difference was found in both aspects (P＜0.05); The percentage of CLA+Ts cell (5.68±10.30)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (5.75±10.19)% of the control group;The percentage of Ts cell express activation-related factors CD38+HLA-DR+was (29.74±26.49)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (25.74±23.60)% of the control group;the percentage of expressing Ki-67+was (7.43±7.38%)%, and no significant difference (P＞0.05) compared with (17.21±19.67)% of the control group.2.2 Effector Ts cellsThe percentage of effector Ts cells (6.64±4.35)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (9.37±8.30)% of the control group. The percentage of effector Ts cell express activation-related factors CD38+HLA-DR+was (8.30±6.70)%in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (5.86±3.98)%of the control group;the percentage of expressing Ki-67+was (6.31±5.76)% , and no significant difference (P＞0.05) when compared to (4.14±3.42)% of the control group. The percentage of CLA+effector Ts cell express activation-related factors CD38+HLA-DR+ was (21.11±15.77)% in psoriatic patients with blood-heat syndrome, and no significant difference (P>0.05) when compared to (18.64±17.39)% of the control group;the percentage of expressing Ki-67+was (9.48±14.56)%, and no significant difference (P>0.05) compared with (9.39±8.95)% of the control group.2.3 Central memory Ts cellsThe percentage of central memory Ts cells (37.80±24.57)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (36.58±22.03)% of the control group. The percentage of central memory Ts cells express activation-related factors CD38+HLA-DR+was (13.52±12.03)% in psoriatic patients with blood-heat syndrome, and no significant difference (P>0.05) when compared to (11.76±11.39)% of the control group;the percentage of expressing Ki-67+was (4.90±5.17)%, and no significant difference (P＞0.05) compared with (3.82±3.33)% of the control group. The percentage of CLA+ central memory Ts cells express activation-related factors CD38+HLA-DR+was (19.26±15.58)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (14.42±13.35)% of the control group;the percentage of expressing Ki-67+was (18.29±14.13)%, and no significant difference (P＞0.05) compared with (15.09±10.31)% of the control group.2.4 Effect memory Ts cellsThe percentage of effect memory Ts cells (33.06±23.13)% in psoriatic patients with blood-heat syndrome, and no significant difference (P＞0.05) when compared to (27.18±19.72)% of the control group. The percentage of central memory T scells express activation-related factors CD38+HLA-DR+was (12.19±10.51)% in psoriatic patients with blood-heat syndrome, and was significantly up-regulated relative to (6.40±5.75)% of the control group, a statistical difference was found in both aspects (P＜0.05);the percentage of expressing Ki-67+was (17.49＋26.52)%, and no significant difference (P＞0.05) compared with (9.53±19.86)% of the control group. The percentage of CLA+central memory Ts cells express activation-related factors CD38+HLA-DR+was (38.68±26.61)% in psoriatic patients with blood-heat syndrome, and was significantly up-regulated relative to (22.92±25.68)% of the control group, a statistical difference was found in both aspects (P＜0.05); the percentage of expressing Ki-67+was (24.78±19.85)%, and was significantly up-regulated relative to (13.15±10.89)% of the control group, a statistical difference was found in both aspects (P＜0.05);ConclusionThe percentage of Tc cells, proliferation levels of effector Tc cells, activation levels of effect memory Tc cells were upregulated, and the percentage of Ts cell were decreased, the activation levels of effect memory Ts cells, activation and proliferation levels of CLA+effect memory Ts cells were all upregulated in the peripheral blood of patients with psoriasis of blood-heat syndrome, suggesting that these cells may participate in the pathogenesis process of blood-heat syndrome of of psoriasis.