| Purpose: 1. To explore the impact of atherosclerosis sclerosis miniature pig model of lipids in brain Aβ as coronary atherosclerosis model Bama miniature pig blood lipids and brain Aβ change. 2.To explore with the spleen expectorant stasis, spleen expectorant, expectorant stasis intervention of Chinese herbal compound on atherosclerosis model of Bama miniature pig brain Aβ from the perspective of anti-lipid peroxidation.Materials and methods:55 6--8 months Canton male Bama miniature pigs were randomly divided into 2 groups: 5 normal group, 50 surgical group. Normal group received the basal diet, high-calorie fat-operation group to be fed the right amount of bile salts, are weighed daily intake of 3% last week, free access to water, adaptive feeding one week. Conducted in the first two weeks of coronary balloon extrusion with injury, the survival of small pigs using random number table 42 surgical group miniature pigs were randomly divided again: the model group(n = 7), spleen group(n = 7), spleen expectorant stasis group(n = 7), spleen expectorant group(n = 7), spleen group(n = 7), expectorant stasis group(n = 7), nitroglycerin group(n = 7), in accordance with each miniature pig feed before feeding, and the addition of relevant treatment compound, mixed in the diet, the model group and the normal group were given the same volume of pure water fed to 24 weeks after surgery Week 0, 24 weeks after surgery, after the pig fasting, taking ear vein to detect changes in blood lipids. And 24 weekend coverage, the miniature pig is fixed on the operating table, anesthesia, open the abdominal cavity, abdominal aortic blood, death, removal of the brain. In this study, normal group selected(n = 5), model group(n = 7), spleen expectorant stasis group(n = 7), spleen expectorant group(n = 7), expectorant stasis group(n = 7) were detected byimmunohistochemical method to detect brain Aβ content, colorimetric detection of serum and brain SOD, GSH-Px activity of. In this study, the results of all the data for statistical analysis using SPSS17.0 results mean ± standard deviation(± S) said correlation analysis using multiple linear regression, between groups were compared using ANOVA.(P <0.05 illustrate statistically significant.)Results: 1. After adaptive feeding pole calorie high-fat diet bile week, Bama miniature pig TC, TG, LDL, HDL were increased, TC increased nearly 2-fold(P <0.01), TG increased nearly 100-fold(P < 0.01), HDL increased nearly 2-fold(P <0.01), LDL-C increased nearly four-fold(P <0.01), prompted during adaptive feeding, miniature pig lipid obvious anomalies, presented dyslipidemia. 2. After 24 weeks treatment, the spleen expectorant stasis group, expectorant stasis group, lipid spleen expectorant action groups mutual differences,(P <0.01), spleen expectorant group regulate TC, HDL, LDL’s ability to expectorant stasis group, spleen expectorant stasis group, spleen expectorant stasis group the ability to regulate TG stronger than spleen expectorant groups and Phlegm stasis group. 3. Compared with normal group, model group, serum, brain tissue SOD, GSH-Px activity decreased(P <0.01); and after treatment Huayu Group spleen expectorant expectorant stasis group, spleen expectorant group was better than Jia(P <0.01); spleen expectorant and Phlegm stasis law, although two different, but not statistically significant(P> 0.05). 4. Spleen Quyu law, spleen expectorant law, expectorant circulation method can reduce brain Aβ levels.(P <0.05) spleen and blood stasis and phlegm method is better than the spleen expectorant law, expectorant circulation method.Conclusion: 1. Coronary atherosclerosis model Bama miniature pigs dyslipidemia, serumanti-lipid peroxidation weakened, spleen expectorant circulation treatment has significant lipid-lowering effect, increased serum lipid peroxidase anti-SOD, GSH-Px activity, shows that the spleen expectorant circulation method has significant lipid-lowering effect, its lipid-lowering effect may be to improve anti-lipid peroxidation to complete. 2. Abnormal lipid content can promote atherosclerosis model Bama mini pig brain Aβ inside, we can see Aβ increase lipid abnormalities may be a risk factor; and spleen expectorant in order to improve the circulation in the brain tissue SOD, GSH-Px activity Aβ-lowering effect may be through improving the mechanism of anti-lipid peroxidation imbalance completed. 3. Spleen expectorant circulation method in lipid, improve anti-lipid peroxidation function method than the spleen expectorant, expectorant circulation method is better described circulation of the spleen phlegm Treatment of dyslipidemia to achieve it good effect, and can prevent Aβ deposition in the brain.
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