| Plants can produce and accumulate reactive oxygen species when they were threatened by living or non-biological factors, as a result, this process will finally cause oxidative stresses in plants. Furthermore, environmental stress on plant tissue culture may also have some important impacts: reactive oxygen species produced during environmental stress will do harm to nucleic acid, protein, cell membrane in tissue culture cells. It's very important to ensure tissue culture cells proliferation by controlling the reactive oxygen species generation - removal mechanism and repairing the damage caused by reactive oxygen species timely and efficiently. On the other hand, environmental stress also has some benifical effects on secondary metabolites accumulation in plants. As one of the important secondary metabolites in grape, resveratrol has been paid more attention because of its significant biological activity, such as anti-microbial, anti-inflammation, anti-cancer, anti-hypertensive, anti-lipid peroxidation and so on. However, like most other useful secondary metabolites, resveratrol content is low. Based on this, we should make breeding selection modifying first during industry production to raise the resveratrol content of natural secondary metabolites in plant cell.In this paper, we studied the dynamic effects on callus growth development and the resveratrol accumulation in tissue culture cells by different treatments: a series of NaCl concentrations(0～0.4mmol/L), the associative action on callus which were treated by NaCl with different precursors and elicitors, different light qualities treatment, we aimd to find the appropriate culture conditions for obtaining the highest resveratrol accumulation and genetic stability callus.The callus induced from the leaves of Vitis Vinifera L."Pinot Noir", which has been sub-cultured for 1 year was used as the material in this experiment, weighing method for growth, organic solvent method for extraction and HPLC method for determination were used to study the influences of different NaCl concentrations culture, different precursors and elicitors with NaCl associative culture, different light quality treatment culture on callus growth dynamic state and resveratrol accumulation. The results showed that:1. Obtaining the highest SOD activity which was 1.54 times higher than distilled water when NaCl concentration was 0.3mol·L-1, meanwhile, resveratrol content reached the highest peak at this concentration, it was 80.76 ug·g-1(DW), 15.7% higher than distilled water treatment. Compared with lower concentration, low NaCl concentration may be more beneficial to CAT activity improvement.2. Separate impacts on POD and CAT activity showed up when NaCl combined with colchicines, phenylalanine, they were improved 1.4 and 1.26 times higher than NaCl treated single, NaCl combined with salicylic, cinnamylate may reduce the activity of POD and CAT, there was no significant influence on tissue culture cell membrane enzyme activities by NaCl withβ-cyclodextrin; NaCl combined with phenylalanine and cinnamylate could improve the content of resveratrol 2.08 and 5.11 times higher separately per unit dry cell, meanwhile, the resveratrol content increased 36.2% under colchicines treatment, but no significant increasing had been observed on resveratrol contents when NaCl combined withβ-cyclodextrin, salicylic.3. The green light could enhance the SOD activity, yellow light may increase the POD and CAT activities, in addition, it may decreased MDA content, the red and blue light reduced the POD, CAT activities. The order of resveratrol accumulation in callus from high to low was: white, yellow, blue, red, green. The resveratrol content was only 41.6637 ug·g-1(DW) when the cell was cultured under green light, it's just 71.6% of white light, yellow was 1.37 times higher than it.
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