| Zanthoxylum dissitum Hemsl is a medical plant which contained various of alkaloid that had significant value in use.In this thesis, we did experiments with the seeds of current year after certain treatment, adopted single-factor, double factors and orthogonal design methods, and studied the effects of the different treatments and sterilization time to the germinate ratio. We analyzed the effects of the proliferation,growth,rooting transplant and cutting caused by the different basal mediums,kinds and concentrations of hormones,substrates and so on. From all the experiments,the main results in this research were as following:(1)Embryo of seeds was the optimal explants which was soaked in 70% alcohol 15s, then transferred into 0.1% HgCl2 8min to sterilize, Embryo was rarely polluted. MS+6-BA 1mg/L+NAA 0.4mg/L was suitable for embryo. The efficiency of callus induction reached 86.67%.(2)The primary culture was MS+ZT2.0mg/L+NAA0.2mg/L, light was 3000～40001x, temperature was 25-30℃, pH was 5.8～6.0, concertration of sucrose was 30g/L.(3)The medium and hormones of proliferation was MS+6-BA0.5mg/L+NAA 0.05mg/L+auxin1.0mg/L. The number of proliferation reached 3.9.(4)Appropriate high temperature was benefited to rooting and 29℃was the optimal temperature for rooting. Roots ware induced on the media of 1/3MS +IBA0.5 mg/L+NAA0.05 mg/L.The percent of rooting reached 78%.(5)The optimum transplant substance was soil+ plant ash+sand (1:1:1) and the ratio of survival reached 80%,The suitable cutting substance was pearlite+ soil (3:1)and the percent of survival reached 49%.The technical rules of propagate Zanthoxylum dissitum Hemsl by tissue culture:treating material→inoculating and induce culture→multiplying axenic plants→culturing strong plants with roots→transplanting and cutting.
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